LOCUS       sequence-112439-         646 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lysosome-targeted FRET biosensor for monitoring Akt activity; 
            targeted to cytosolic face of lysosomal membrane..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Zhou X, Clister TL, Lowry PR, Seldin MM, Wong GW, Zhang J
  TITLE     Dynamic Visualization of mTORC1 Activity in Living Cells.
  JOURNAL   Cell Rep. 2015 Mar 11. pii: S2211-1247(15)00177-1. doi: 
            10.1016/j.celrep.2015.02.031.
  PUBMED    25772363
REFERENCE   2  (bases 1 to 646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2015 Mar 11. pii: S2211-1247(15)00177-1. doi: 
            10.1016/j.celrep.2015.02.031."
COMMENT     Sequence Label: pcDNA3-Lyso-AktAR2
FEATURES             Location/Qualifiers
     source          1..646
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      85..94
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      89..98
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttgc ggccgccacc atggcggcgc ccggcagcgc ccggcgaccc
      121 ctgctgctgc tactgctgtt gctgctgctc ggcctcatgc attgtgcgtc agcagcaatg
      181 tttatggtga aaaatggcaa cgggaccgcg tgcataatgg ccaacttctc tgctgccttc
      241 tcagtgaact acgacaccaa gagtggccct aagaacatga cctttgacct gccatcagat
      301 gccacagtgg tgctcaaccg cagctcctgt ggaaaagaga acacttctga ccccagtctc
      361 gtgattgctt ttggaagagg acatacactc actctcaatt tcacgagaaa tgcaacacgt
      421 tacagcgtcc agctcatgag ttttgcttat aacttgtcag acacacacct tttccccaat
      481 gcgagctcca aagaaatcaa gactgtggaa tctataactg acatcagggc agatatagat
      541 aaaaaataca gatgtgttag tggcacccag gtccacatga acaacgtgac cgtaacgccc
      601 catgatgcca ccatccaggc gtacctttcc aacagcagct tcagca
//
