LOCUS       sequence-112445-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi/ER, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-ELP-1-25
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      679..688
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(720..771)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(749..769)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tcgactgcag aattcgaagc ttgagctccc ccccaccgcc tgctggcaaa ctgagcttct
       61 ttcccttgag tacttttgta atgtacaagt agaagaagtc acagtatagg atggtctgga
      121 ctacgccggc caccacagca atgaggtcaa agaagccctc aaagtagaag cgccagatcc
      181 agttgacaag atacaaagca cgatagaggc ccaggaagaa caggtagtgg gtggtgatgg
      241 tctcggcctc cccagtcttg ctgatcataa acagctgcgg aaggatagcc acggactcca
      301 ggtagatgga gaaggtccag aggatctcaa gaggagagaa atcgtgatta actaaaaatg
      361 agaggcctcc cacagggacc accagaaact ccactcggaa ggtatcatga tttccatcgt
      421 aggttgcctt aaatttcagg tagatcaggt acactgtggc ataggagcag gcaaggtaga
      481 taaccttcat agatgtgtta tacaatgaaa taaatgaagt aaaaagatcc aggtaacgag
      541 ttgtgaagac cagtgcaaac agaagctggc ttttcccaga aataccggcg caggagcgcg
      601 tcttccagat cttcagcagc aggatgacga tggccgccag gtgggacagg tccccagtca
      661 gccggaaaat gttcatggcc atggtggcgg cgctagcgga tctgacggtt cactaaacca
      721 gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg
//
