LOCUS       sequence-112455-         705 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cytosol-targeted, FRET-based PKA activity reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Herbst KJ, Allen MD, Zhang J
  TITLE     Spatiotemporally regulated protein kinase A activity is a critical 
            regulator of growth factor-stimulated extracellular signal-regulated
            kinase signaling in PC12 cells.
  JOURNAL   Mol Cell Biol. 2011 Oct;31(19):4063-75. doi: 10.1128/MCB.05459-11. 
            Epub 2011 Aug 1.
  PUBMED    21807900
REFERENCE   2  (bases 1 to 705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.05459-11"; journalName: "Mol Cell Biol"; date: 
            "2011-10"; volume: "31"; issue: "19"; pages: "4063-75"
COMMENT     Sequence Label: pcDNA3-AKAR4-NES
FEATURES             Location/Qualifiers
     source          1..705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(10..36)
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             complement(join(43..555,556..558,559..561))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     236..255
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     495..516
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(622..643)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tctagattac agggtcaggc gctccagggg gggcaggaat tcctcgatgt tgtggcggat
       61 cttgaagttg gccttgatgc cgttcttctg cttgtcggcg gtgatataga cgttgtggct
      121 gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc
      181 cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt
      241 gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc
      301 ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cgggcgaagc actgaaggcc
      361 gtagcccagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat
      421 cagcttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa
      481 cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc cggtgaacag
      541 ctcctcgccc ttgctcacca tagatccacc ggtacctccc ttgtacagct cgtccatgcc
      601 gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt
      661 ggggtctttg ctcagcttgg actggtagct caggtagtgg ttgtc
//
