LOCUS       sequence-112456-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Envelope, Excitation: 513, Emission: 527.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: YPet-LaminB1-10
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(46..48,49..51,52..762)
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELYK"
     primer_bind     complement(352..371)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tggtttagtg aaccgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaaaggc
       61 gaagagctgt tcaccggcgt ggtgcccatc ctggtggagc tggacggcga cgtgaacggc
      121 cacaagttca gcgtgagcgg cgagggcgag ggcgacgcca cctacggcaa gctgaccctg
      181 aagctgctgt gcaccaccgg caagctgccc gtgccctggc ccaccctggt gaccaccctg
      241 ggctacggcg tgcagtgctt cgcccggtac cccgaccaca tgaagcagca cgacttcttc
      301 aagagcgcca tgcccgaggg ctacgtgcag gagcggacca tcttcttcaa ggacgacggc
      361 aactacaaga cccgggccga ggtgaagttc gagggcgaca ccctggtgaa ccggatcgag
      421 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg gccacaagct ggagtacaac
      481 tacaacagcc acaacgtgta catcaccgcc gacaagcaga agaacggcat caaggccaac
      541 ttcaagatcc ggcacaacat cgaggacggc ggcgtgcagc tggccgacca ctaccagcag
      601 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag
      661 agcgccctgt tcaaggaccc caacgagaag cgggaccaca tggtgctgct ggagttcctg
      721 accgccgccg gcatcaccga gggcatgaac gagctctata agtccggact cagatctcga
      781 gctcaagctt cgatggcgac tgcgaccccc gtgccgccgc
//
