LOCUS       sequence-112457-         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET-based biosensor for monitoring calcineurin (CaN) phosphatase 
            activity..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Mehta S, Aye-Han NN, Ganesan A, Oldach L, Gorshkov K, Zhang J
  TITLE     Calmodulin-controlled spatial decoding of oscillatory Ca2+ signals 
            by calcineurin.
  JOURNAL   eLife. 2014 Jul 23;3:e03765. doi: 10.7554/eLife.03765.
  PUBMED    25056880
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2014-07-23"; volume: "3:e03765. doi"; pages: " 
            10.7554/eLife.03765"
COMMENT     Sequence Label: pcDNA3-CaNAR2
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(58..768)
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKL
                     LCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLEF
                     LTAAGITEGMNELYK"
ORIGIN
        1 tctagatgca tgctcgagcg gccgccagtg tgatggatat ctgcagaatt cttactattt
       61 gtacaattca ttcataccct cggtaatacc agcagcagtc aaaaattcta acaagaccat
      121 gtggtctctc ttttcgtttg gatctttgaa taaggcagat tgataggata agtaatggtt
      181 gtctggtaac aagactggac catcaccaat tggagtattt tgttgataat ggtcagctaa
      241 ttgaacacca ccatcttcaa tgttgtgtct aattttgaag ttagctttga taccattctt
      301 ttgtttgtca gcagtgatgt aaacattgtg agagttatag ttgtattcca atttgtgacc
      361 taaaatgtta ccatcttctt taaaatcaat accttttaat tcgattctat taactaaggt
      421 atcaccttca aacttgactt cagctctggt cttgtagtta ccgtcatctt tgaaaaaaat
      481 agttctttct tgaacataac cttctggcat ggcagacttg aaaaagtcat gttgtttcat
      541 atgatctggg tatctagcaa aacattgaac accataacct aaagtagtga ctaaggttgg
      601 ccatggaact ggcaatttac cagtagtaca gagtaatttt aaggtcaatt taccgtacgt
      661 agcatcacct tcaccttcac cggagacaga aaatttgtga ccattaacat caccatctaa
      721 ttcaaccaaa attgggacaa caccagtgaa taattcttca cctttagaga gctcaaca
//
