LOCUS       sequence-112458-         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) 
            phosphatase activity..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Mehta S, Aye-Han NN, Ganesan A, Oldach L, Gorshkov K, Zhang J
  TITLE     Calmodulin-controlled spatial decoding of oscillatory Ca2+ signals 
            by calcineurin.
  JOURNAL   eLife. 2014 Jul 23;3:e03765. doi: 10.7554/eLife.03765.
  PUBMED    25056880
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2014-07-23"; volume: "3:e03765. doi"; pages: " 
            10.7554/eLife.03765"
COMMENT     Sequence Label: pcDNA3-Cyto-CaNAR2
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(10..36)
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     primer_bind     434..453
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tctagattac agggtcaggc gctccagggg gggcaggaat tctttgtaca attcattcat
       61 accctcggtg atgccggcgg cggtcaggaa ctccagcagc accatgtggt cccgcttctc
      121 gttggggtcc ttgaacaggg cgctctggta gctcaggtag tggttatcgg gcagcagcac
      181 ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gccagctgca cgccgccgtc
      241 ctcgatgttg tgccggatct tgaagttggc cttgatgccg ttcttctgct tgtcggcggt
      301 gatgtacacg ttgtggctgt tgtagttgta ctccagcttg tggcccagga tgttgctgtc
      361 ctccttgaag tcgatgccct tcagctcgat ccggttcacc agggtgtcgc cctcgaactt
      421 cacctcggcc cgggtcttgt agttgccgtc gtccttgaag aagatggtcc gctcctgcac
      481 gtagccctcg ggcatggcgc tcttgaagaa gtcgtgctgc ttcatgtggt cggggtaccg
      541 ggcgaagcac tgcacgccgt agcccagggt ggtcaccagg gtgggccagg gcacgggcag
      601 cttgccggtg gtgcacagca gcttcagggt cagcttgccg taggtggcgt cgccctcgcc
      661 ctcgccgctc acgctgaact tgtggccgtt cacgtcgccg tccagctcca ccaggatggg
      721 caccacg
//
