LOCUS       sequence-112470-         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) 
            activation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Mehta S, Aye-Han NN, Ganesan A, Oldach L, Gorshkov K, Zhang J
  TITLE     Calmodulin-controlled spatial decoding of oscillatory Ca2+ signals 
            by calcineurin.
  JOURNAL   eLife. 2014 Jul 23;3:e03765. doi: 10.7554/eLife.03765.
  PUBMED    25056880
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2014-07-23"; volume: "3:e03765. doi"; pages: " 
            10.7554/eLife.03765"
COMMENT     Sequence Label: pcDNA3.1(+)-Cyto-CaNARi
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(10..720,721..723,724..726))
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(52..73)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     401..420
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     660..681
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 caggaattcc ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc
       61 cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagcttgg actggtagct
      121 caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta
      181 gtggtcggcg agctgcacgc cgccgtcctc gatgttgtgg cggatcttga agttggcctt
      241 gatgccgttc ttctgcttgt cggcggtgat atagacgttg tggctgttgt agttgtactc
      301 cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg
      361 gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc
      421 cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc
      481 gtgctgcttc atgtggtcgg ggtagcgggc gaagcactgc aggccgtagc ccagggtggt
      541 cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatcagct tcagggtcag
      601 cttgccgtag gtggcatcgc cctcgccctc gccggacacg ctgaacttgt ggccgtttac
      661 gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct
      721 caccatctcg agctgaatat tgctgctatt actgccattg ctgtccgtgc cgttagtctc
      781 tgaggtgaga g
//
