LOCUS       sequence-112483-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Cas9 as a Type 3 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK036
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      104..138
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(161..181)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(185..205)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cagtactgcg atgagtggca gggcggggcg taatttgata tcgagctcgc ttggactcct
       61 gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg
      121 ccgccgggcg ttttttattg gtctggtctc aggatcctta tacctttctc ttcttttttg
      181 gatctacctt tctcttcttt tttggatcag ctccctcatc ccctccgagc tgtgagaggt
      241 caatccgagt ttcgtaaagt ccggtgatgc tctggtggat gagggtggcg tccagcactt
      301 ctttggtgga ggtatagcgt ttccggtcga tggtggtgtc gaagtacttg aatgcggcag
      361 gggcaccaag attggtgagg gtgaacaggt ggatgatatt ctcagcctgc tcccgaattg
      421 gtttgtctct gtgcttattg tatgcgctaa ggactttatc gaggttggca tcagcgagaa
      481 tgacgcgttt agaaaactcg gaaatctgct caatgatctc gtccagataa tgcttatgct
      541 gctccacaaa cagttgcttt tgctcgttgt cctcggggct ccccttcagc ttctcgtaat
      601 ggcttgcaag gtagaggaaa ttcacgtact tggaggggag agccagctca ttacccttct
      661 gaagttcgcc agcggaggcg agcatccgct tccgcccatt ctccagttcg aacaggctat
      721 actttgggag cttgatgata aggtccttct tgacttcctt gtaacctttg g
//
