LOCUS       sequence-112484-         386 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lim Lab plasmid: AmpR + PconNoHindM8::micC::Asp terminator + 
            PLlacO-1::T710::hfq::T1 terminator + PLtetO-1m9::ompC::gfp::T1T2 
            terminator + ColE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Sagawa S, Shin JE, Hussein R, Lim HN
  TITLE     Paradoxical suppression of small RNA activity at high Hfq 
            concentrations due to random-order binding.
  JOURNAL   Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
  PUBMED    26261213
REFERENCE   2  (bases 1 to 386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Aug 10. pii: gkv777."
COMMENT     Sequence Label: pHL2107
FEATURES             Location/Qualifiers
     source          1..386
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    133..151
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    158..176
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(197..215)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        283..386
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catagctggg accaggaggg acagtacttt aactttcatg ttattaaccc tctgttatat
       61 gcctttattt gcttttttat gccactgcat actgattaac cctcattaat cagtcggcaa
      121 gtcggacaga tctctctatc actgataggg atgtcaatct ctatcactga tagggactcg
      181 aggtgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
      241 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
      301 ttattttnct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
      361 cttcaataat attgaaaaag gaagag
//
