LOCUS       sequence-112489-        1043 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Venus as a Type 3a part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK038
FEATURES             Location/Qualifiers
     source          1..1043
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      100..134
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(154..867)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEGGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 actgcgatga gtggcagggc ggggcgtaat ttgatatcga gctcgcttgg actcctgttg
       61 atagatccag taatgacctc agaactccat ctggatttgt tcagaacgct cggttgccgc
      121 cgggcgtttt ttattggtct ggtctcaaga acctttgtac aattcatcca taccatgggt
      181 aataccagca gcagtaacaa attctaacaa gaccatgtga tctctctttt cgtttggatc
      241 tttggataag gcagattgat aggataagta atggttgtct ggtaacaaga ctggaccatc
      301 accaattgga gtattttgtt gataatggtc agctaattga acaccaccat cttcaatgtt
      361 gtgtctaatt ttgaagttag ctttgatacc attcttttgt ttgtcagcag tgatgtaaac
      421 attgtgagag ttatagttgt attccaattt gtgacctaaa atgttaccac cttctttaaa
      481 atcaatacct tttaattcga ttctattaac taaggtatca ccttcaaact tgacttcagc
      541 tctggtcttg tagttaccgt catctttgaa aaaaatagtt ctttcttgaa cataaccttc
      601 tggcatggca gacttgaaaa agtcatgttg tttcatatga tctgggtatc tagcaaaaca
      661 ttgcaaacca taacctaaag tagtgactaa ggttggccat ggaactggca atttaccagt
      721 agtacaaatc aattttaagg tcaatttacc gtaagtagca tcaccttcac cttcaccgga
      781 gacagaaaat ttgtgaccat taacatcacc atctaattca accaaaattg ggacaacacc
      841 agtgaataat tcttcacctt tagacatatg agaccgacta cggttatcca cagaatcagg
      901 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
      961 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     1021 acgctcaagt cagaggtggc gaa
//
