LOCUS       sequence-112490-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lim Lab plasmid: AmpR + PLlacO-1::T710 gfp::Asp terminator + ColE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Sagawa S, Shin JE, Hussein R, Lim HN
  TITLE     Paradoxical suppression of small RNA activity at high Hfq 
            concentrations due to random-order binding.
  JOURNAL   Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
  PUBMED    26261213
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Aug 10. pii: gkv777."
COMMENT     Sequence Label: pHL1633
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(62..82)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     677..705
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     CDS             complement(745..777)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     RBS             785..807
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cctgtacgat taaagcctta tttgtatagt tcatccatgc catgtgtaat cccagtcagc
       61 tgttacaaac tcaagaagga ccatgtggtc tctcttttcg ttgggatctt tcgaaagtgc
      121 agattgtgtg gacaggtaat ggttgtctgg taaaaggaca gggccatcgc caattggagt
      181 attttgttga taatggtctg ctagttgaac gcttccatct tcaatgttgt gtctaatttt
      241 gaagttaact ttgattccat tcttttgttt gtctgccatg atgtatacat tgtgtgagtt
      301 atagttgtat tccaatttgt gtccaagaat gtttccatct tctttaaaat caataccttt
      361 taactcgatt ctattaacaa gggtatcacc ttcaaacttg acttcagcac gtgtcttgta
      421 gttcccgtca tctttgaaaa atatagttct ttcctgtaca taaccttcgg gcatggcact
      481 cttgaaaaag tcatgctgtt tcatatgatc tgggtatctc gcaaagcatt gaacaccata
      541 accgaaagta gtgacaagtg ttggccatgg aacaggtagt tttccagtag tgcaaataaa
      601 tttaagggta agttttccgt atgttgcatc accttcaccc tctccactga cagaaaattt
      661 gtgcccatta acatcaccat ctaattcaac aagaattggg acaactccag tgaaaagttc
      721 ttctccttta cgcatggatc cgcgacccat ttgctgtcca ccagtcatgc tagccatatg
      781 tatatctcct tcttaaagtt aaacaaaatt atttctagag gtaccagtat cttgttatcc
      841 gctcacattt atggatcagt cgacctgcag ttcgagacgt ca
//
