LOCUS       sequence-112493-         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AmpR + ColE1 + PLtetO-1::RBS(st7):cro::cII (31 basepairs)::T1 
            terminator + PLlacO-1::RBS(st7):psu::T1 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Hussein R, Lee TY, Lim HN
  TITLE     Quantitative characterization of gene regulation by Rho dependent 
            transcription termination.
  JOURNAL   Biochim Biophys Acta. 2015 May 14;1849(8):940-954. doi: 
            10.1016/j.bbagrm.2015.05.003.
  PUBMED    25982507
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbagrm.2015.05"; journalName: "Biochim Biophys Acta"; 
            date: "2015-05-14- 14"; volume: "1849"; issue: "8"; pages: "940-954"
COMMENT     Sequence Label: pHL 2057
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     77..95
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    116..134
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    141..159
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      579..665
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    716..732
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
       61 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcacc tcgagtccct
      121 atcagtgata gagattgaca tccctatcag tgatagagat actggtacct aaggaggaaa
      181 aaaaatggaa caacgcataa ccctgaaaga ttatgcaatg cgctttgggc aaaccaagac
      241 agctaaagat ctcggcgtat atcaaagcgc gatcaacaag gccattcatg caggccgaaa
      301 gattttttta actataaacg ctgatggaag cgtttatgcg gaagaggtaa agcccttccc
      361 gagtaacaaa aaaacaacag cataaataac cccgctctta cacattccag ccctgaaaaa
      421 gggcatcaaa ttaaaccaca cctatggtgt atgcatttat ttgcatacat tcaatcaatt
      481 gttatctaag gaaatactta catatggttc gtgcaaacaa acgcaacgag gctctataag
      541 gatcctatga agcttcatgg tacgcgtgct agaggcatca aataaaacga aaggctcagt
      601 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
      661 caaatccgcc gccctagaga attcgatcca taaatgtgag cggataacat tgacattgtg
      721 agcggataac aagatactcc cgggtaagga ggaaaaaaaa tggaaagcac agccttacag
      781 caggcctttg acacctgtca gaataacaaa gcagcatggc tgcaacgcaa aaatgagctg
      841 gcagcggccg aacaggaata tctgcggctt ctgtcaggag aaggcagaaa cgtcagtcgc
      901 ctggacg
//
