LOCUS       sequence-112495-         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AmpR + ColE1 + PLtetO-1:: extra 5'UTR::RBS(st7):cfp::T1 terminator +
            PLlacO-1::RBS(st7):psu::T1 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Hussein R, Lee TY, Lim HN
  TITLE     Quantitative characterization of gene regulation by Rho dependent 
            transcription termination.
  JOURNAL   Biochim Biophys Acta. 2015 May 14;1849(8):940-954. doi: 
            10.1016/j.bbagrm.2015.05.003.
  PUBMED    25982507
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbagrm.2015.05"; journalName: "Biochim Biophys Acta"; 
            date: "2015-05-14- 14"; volume: "1849"; issue: "8"; pages: "940-954"
COMMENT     Sequence Label: pHL 2067
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     78..96
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    117..135
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    142..160
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             join(213..215,216..218,219..731)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     primer_bind     complement(258..279)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(519..538)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     866..887
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
       61 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcac ctcgagtccc
      121 tatcagtgat agagattgac atccctatca gtgatagaga tactggtacc taatagaaat
      181 aattttgttt aactttataa ggaggaaaaa aaatggtgag caagggcgag gagctgttca
      241 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aggttcagcg
      301 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      361 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tggggcgtgc
      421 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      481 ccgaaggcta cgtccaggag cgtaccatct tcttcaagga cgacggcaac tacaagaccc
      541 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
      601 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac atcagccaca
      661 acgtctatat caccgccgac aagcagaaga acggcatcaa ggcccacttc aagatccgcc
      721 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
      781 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca
      841 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
      901 tcactcacgg catgga
//
