LOCUS       sequence-112496-        1003 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes 6XHis_3XFlag as a Type 3a part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK040
FEATURES             Location/Qualifiers
     source          1..1003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      104..138
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(158..175)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(179..250)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     rep_origin      complement(365..953)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(445..464)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cagtactgcg atgagtggca gggcggggcg taatttgata tcgagctcgc ttggactcct
       61 gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg
      121 ccgccgggcg ttttttattg gtctggtctc aagaaccgtg atgatgatga tgatgacctt
      181 tatcatcatc gtccttataa tctttgtcat catcatcttt gtagtcctta tcatcatcgt
      241 ccttgtaatc catatgagac cgactacggt tatccacaga atcaggggat aacgcaggaa
      301 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
      361 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
      421 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
      481 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
      541 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
      601 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
      661 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
      721 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
      781 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
      841 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
      901 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
      961 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaa
//
