LOCUS       sequence-112500-         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  KanR + ColE1 + 
            PLlacO-1::RBS(st7):rfp::RBS(st7):cfp::RBS(st7):yfp::T1 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Hussein R, Lee TY, Lim HN
  TITLE     Quantitative characterization of gene regulation by Rho dependent 
            transcription termination.
  JOURNAL   Biochim Biophys Acta. 2015 May 14;1849(8):940-954. doi: 
            10.1016/j.bbagrm.2015.05.003.
  PUBMED    25982507
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbagrm.2015.05"; journalName: "Biochim Biophys Acta"; 
            date: "2015-05-14- 14"; volume: "1849"; issue: "8"; pages: "940-954"
COMMENT     Sequence Label: pHL 309
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    215..231
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(404..422)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     672..691
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtgcatccac cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcagatctt
       61 gatcccctgc gccatcagat ccttggcggc aagaaagcca tccagtttac tttgcagggc
      121 ttcccaacct taccagaggg cgccccagct ggcaattccg acgtctcgaa ctgcaggtcg
      181 actgatccat aaatgtgagc ggataacatt gacattgtga gcggataaca agatactggt
      241 acctaaggag gaaaaaaaat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
      301 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
      361 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
      421 aagggcggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
      481 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
      541 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
      601 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
      661 ccctccgacg gccccgtaat gcagaagaag acaatgggct gggaggcctc ctccgagcgg
      721 atgtaccccg aggacggcgc c
//
