LOCUS       sequence-112505-         347 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for expressing U6 sgRNA and CAGGS Cerulean 
            fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin 
            S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, 
            Davidsohn N, Housden BE, Perrimon N, Weiss R, Aach J, Collins JJ, 
            Church GM
  TITLE     Highly efficient Cas9-mediated transcriptional programming.
  JOURNAL   Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312.
  PUBMED    25730490
REFERENCE   2  (bases 1 to 347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312."
COMMENT     Sequence Label: pSB700
FEATURES             Location/Qualifiers
     source          1..347
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     81..102
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      144..153
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(154..173)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(235..257)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 cagaatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac
       61 gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt
      121 gaacagctcc tcgcccttgc tcaccatggt ggccacaata accagcacgt tgcccaggag
      181 ctgtaggaaa aagaagaagg catgaacatg gttagcagag gctctagagc cgccggtcac
      241 acgccagaag ccgaaccccg ccctgccccg tcccccccga aggcagccgt ccccccgcgg
      301 acagccccga ggctggagag ggagaagggg acggcggcgc ggcgacg
//
