LOCUS       sequence-112506-         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Venus as a Type 3b part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK045
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      101..135
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(155..865)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKL
                     ICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEGGNILGHKLEYNYNSHNVYITADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
ORIGIN
        1 tactgcgatg agtggcaggg cggggcgtaa tttgatatcg agctcgcttg gactcctgtt
       61 gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg
      121 ccgggcgttt tttattggtc tggtctcagg atcctttgta caattcatcc ataccatggg
      181 taataccagc agcagtaaca aattctaaca agaccatgtg atctctcttt tcgtttggat
      241 ctttggataa ggcagattga taggataagt aatggttgtc tggtaacaag actggaccat
      301 caccaattgg agtattttgt tgataatggt cagctaattg aacaccacca tcttcaatgt
      361 tgtgtctaat tttgaagtta gctttgatac cattcttttg tttgtcagca gtgatgtaaa
      421 cattgtgaga gttatagttg tattccaatt tgtgacctaa aatgttacca ccttctttaa
      481 aatcaatacc ttttaattcg attctattaa ctaaggtatc accttcaaac ttgacttcag
      541 ctctggtctt gtagttaccg tcatctttga aaaaaatagt tctttcttga acataacctt
      601 ctggcatggc agacttgaaa aagtcatgtt gtttcatatg atctgggtat ctagcaaaac
      661 attgcaaacc ataacctaaa gtagtgacta aggttggcca tggaactggc aatttaccag
      721 tagtacaaat caattttaag gtcaatttac cgtaagtagc atcaccttca ccttcaccgg
      781 agacagaaaa tttgtgacca ttaacatcac catctaattc aaccaaaatt gggacaacac
      841 cagtgaataa ttcttcacct ttagaagaat gagaccgact acgg
//
