LOCUS       sequence-112510-         987 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses shRNA targeting lnc-BATE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Alvarez-Dominguez JR, Bai Z, Xu D, Yuan B, Lo KA, Yoon MJ, Lim YC, 
            Knoll M, Slavov N, Chen S, Chen P, Lodish HF, Sun L
  TITLE     De Novo Reconstruction of Adipose Tissue Transcriptomes Reveals Long
            Non-coding RNA Regulators of Brown Adipocyte Development.
  JOURNAL   Cell Metab. 2015 May 5;21(5):764-76. doi: 
            10.1016/j.cmet.2015.04.003. Epub 2015 Apr 23.
  PUBMED    25921091
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2015.04.003"; journalName: "Cell Metab"; date: 
            "2015-05-5- 5"; volume: "21"; issue: "5"; pages: "764-76"
COMMENT     Sequence Label: PMKO-sh_lnc-BATE1
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        62..310
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     62..82
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     233..252
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(395..415)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        400..729
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      580..715
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(738..754)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(739..755)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 cagccctcac tccttctcta ggcgccggaa ttgaagatct gtcgacaagg tcgggcagga
       61 agagggccta tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga
      121 gataattaga attaatttga ctgcaaacac aaagatatta gtacaaaata cgtgacgtag
      181 aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca
      241 tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg
      301 acgaaacacc gggccttcct ttagacttga cctctcgaga ggtcaagtct aaaggaaggc
      361 tttttgaatt cgccagcaca gtggtcgacc ctgtggaatg tgtgtcagtt agggtgtgga
      421 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
      481 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
      541 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
      601 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
      661 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
      721 ttttgcaaaa agcttatcga taccgtcgac ctcgagacca tggtgagcaa gcagatcctg
      781 aagaacaccg gcctgcagga gatcatgagc ttcaaggtga acctggaggg cgtggtgaac
      841 aaccacgtgt tcaccatgga gggctgcggc aagggcaaca tcctgttcgg caaccagctg
      901 gtgcagatcc gcgtgaccaa gggcgccccc ctgcccttcg ccttcgacat cctgagcccc
      961 gccttccagt acggcaaccg caccttc
//
