LOCUS       sequence-112513-         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Backbone pComb3X containing PCR template for human antibody lambda 
            light chain constant region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Andris-Widhopf J, Rader C, Steinberger P, Fuller R, Barbas CF 3rd
  TITLE     Methods for the generation of chicken monoclonal antibody fragments 
            by phage display.
  JOURNAL   J Immunol Methods. 2000 Aug 28;242(1-2):159-81.
  PUBMED    10986398
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Immunol 
            Methods."; date: "2000-08-28"; volume: "242(1-2)"; pages: "159-81"
COMMENT     Sequence Label: pComb3XLambda
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             697..714
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             721..747
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cagtctgggg ctgaggtgaa gaagcctggg tcctcggtga aggtctcctg cagggcttct
       61 ggaggcacct tcaacaatta tgccatcagc tgggtgcgac aggcccctgg acaagggctt
      121 gagtggatgg gagggatctt ccctttccgt aatacagcaa agtacgcaca acacttccag
      181 ggcagagtca ccattaccgc ggacgaatcc acgggcacag cctacatgga gctgagcagc
      241 ctgagatctg aggacacggc catatattat tgtgcgagag gggatacgat ttttggagtg
      301 accatgggat actacgctat ggacgtctgg ggccaaggga ccacggtcac cgtctccgca
      361 gcctccacca agggcccatc ggtcttcccc ctggcaccct cctccaagag cacctctggg
      421 ggcacagcgg ccctgggctg cctggtcaag gactacttcc ccgaaccggt gacggtgtcg
      481 tggaactcag gcgccctgac cagcggcgtg cacaccttcc cggctgtcct acagtcctca
      541 ggactctact ccctcagcag cgtggtgacc gtgccctcca gcagcttggg cacccagacc
      601 tacatctgca acgtgaatca caagcccagc aacaccaagg tggacaagaa agttgagccc
      661 aaatcttgtg acaaaactag tggccaggcc ggccagcacc atcaccatca ccatggcgca
      721 tacccgtacg acgttccgga ctacgcttct ta
//
