LOCUS       sequence-112523-        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a scrambled shRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Alvarez-Dominguez JR, Bai Z, Xu D, Yuan B, Lo KA, Yoon MJ, Lim YC, 
            Knoll M, Slavov N, Chen S, Chen P, Lodish HF, Sun L
  TITLE     De Novo Reconstruction of Adipose Tissue Transcriptomes Reveals Long
            Non-coding RNA Regulators of Brown Adipocyte Development.
  JOURNAL   Cell Metab. 2015 May 5;21(5):764-76. doi: 
            10.1016/j.cmet.2015.04.003. Epub 2015 Apr 23.
  PUBMED    25921091
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2015.04.003"; journalName: "Cell Metab"; date: 
            "2015-05-5- 5"; volume: "21"; issue: "5"; pages: "764-76"
COMMENT     Sequence Label: PMKO-shNC
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        63..311
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     63..83
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     234..253
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(396..416)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        401..730
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      581..716
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(739..755)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(740..756)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 ccagccctca ctccttctct aggcgccgga attgaagatc tgtcgacaag gtcgggcagg
       61 aagagggcct atttcccatg attccttcat atttgcatat acgatacaag gctgttagag
      121 agataattag aattaatttg actgcaaaca caaagatatt agtacaaaat acgtgacgta
      181 gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc
      241 atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
      301 gacgaaacac cggcaacaag atgaagagca ccaactcgag ttggtgctct tcatcttgtt
      361 gtttttgaat tcgccagcac agtggtcgac cctgtggaat gtgtgtcagt tagggtgtgg
      421 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
      481 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
      541 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
      601 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
      661 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
      721 cttttgcaaa aagcttatcg ataccgtcga cctcgagacc atggtgagca agcagatcct
      781 gaagaacacc ggcctgcagg agatcatgag cttcaaggtg aacctggagg gcgtggtgaa
      841 caaccacgtg ttcaccatgg agggctgcgg caagggcaac atcctgttcg gcaaccagct
      901 ggtgcagatc cgcgtgacca agggcgcccc cctgcccttc gccttcgaca tcctgagccc
      961 cgccttccag tacggcaacc gcaccttcac caagtccgag
//
