LOCUS       sequence-112536-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of 6xHis-(+36)GFP in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Zuris JA, Thompson DB, Shu Y, Guilinger JP, Bessen JL, Hu JH, Maeder
            ML, Joung JK, Chen ZY, Liu DR
  TITLE     Cationic lipid-mediated delivery of proteins enables efficient 
            protein-based genome editing in vitro and in vivo.
  JOURNAL   Nat Biotechnol. 2015 Jan;33(1):73-80. doi: 10.1038/nbt.3081. Epub 
            2014 Oct 30.
  PUBMED    25357182
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3081"; 
            journalName: "Nat Biotechnol"; date: "2015-01"; volume: "33"; issue:
            "1"; pages: "73-80"
COMMENT     Sequence Label: pET-6xHis-(pos36)GFP
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..50)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(798..815)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             828..850
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    865..889
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gtagacgagt ccatgtgctg gcgttcaaat ttcgcagcag cggtttcttt accagactcg
       61 agggtacctc attattactt gtagcgttcg tcgcgtccgt gcttaatgcc tgcagcggtc
      121 acgaattcta acagaaccat gtgatcgcgt ttttctttcg gatctttgct cagtttagaa
      181 cgggtgctca ggtagtggtt gcgaggtaac agtacaggcc cgcgaccaat cggcgtgttt
      241 tgttgataat gatccgctaa ttgaacgctg ccgtctttca cgttatggcg aattttgaat
      301 tttgccttga taccattctt gcgtttatcg gccgtaatat acaccttatg agaattaaag
      361 ttataacgca gtttatggcc taaaatgttg cctttttctt tgaagtcacg acctttcagt
      421 ttgatacgat ttactaatgt gcggccttca aatttcactt ccgcacgcgt tttatatttg
      481 ccatccttct tgaagctgat tgtacgctct tgaacatatc ctttaggcat ggctgatttg
      541 aaaaaatcgt gacgtttcat atgtttaggg taacgactga agcactgaac gccataggtc
      601 agggtggtca ctaaggtggg ccacggaacg ggtaatttgc ctgttgtgca aataaacttc
      661 agggtcaatt taccacgggt agcgtcacct ttgcctttgc cgcgcacgct aaatttatga
      721 ccgttcacgt cgccctttaa ttccactaag atcggtactt taccacgaaa cagacgttca
      781 cctttgctag cgccaccgtg atggtggtgg tgatgaccca tggtatatct ccttcttaaa
      841 gttaaacaaa attatttcta gaggggaatt gttatccgct cacaattccc
//
