LOCUS       sequence-112538-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of (+36)GFP-GGS9-Cre-6xHis in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Zuris JA, Thompson DB, Shu Y, Guilinger JP, Bessen JL, Hu JH, Maeder
            ML, Joung JK, Chen ZY, Liu DR
  TITLE     Cationic lipid-mediated delivery of proteins enables efficient 
            protein-based genome editing in vitro and in vivo.
  JOURNAL   Nat Biotechnol. 2015 Jan;33(1):73-80. doi: 10.1038/nbt.3081. Epub 
            2014 Oct 30.
  PUBMED    25357182
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3081"; 
            journalName: "Nat Biotechnol"; date: "2015-01"; volume: "33"; issue:
            "1"; pages: "73-80"
COMMENT     Sequence Label: pET-(pos36)GFP-9xGGS-Cre-6xHis
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..50)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(69..86)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gtagacgagt ccatgtgctg gcgttcaaat ttcgcagcag cggtttcttt accagactcg
       61 agtcattagt gatggtggtg gtgatgggat ccgccgtcgc catcttccag caggcgcacc
      121 attgcccctg tttcactatc caggttacgg atatagttca tgacaatatt tacattggtc
      181 cagccaccag cttgcatgat ctccggtatt gaaactccag cgcgggccat atctcgcgcg
      241 gctccgacac gggcactgtg tccagaccag gccaggtatc tctgaccaga gtcatcctta
      301 gcgccgtaaa tcaatcgatg agttgcttca aaaatccctt ccagggcgcg agttgatagc
      361 tggctggtgg cagatggcgc ggcaacacca ttttttctga cccggcaaaa caggtagtta
      421 ttcggatcat cagctacacc agagacggaa atccatcgct cgaccagttt agttaccccc
      481 aggctaagtg ccttctctac acctgcggtg ctaaccagcg ttttcgttct gccaatatgg
      541 attaacattc tcccaccgtc agtacgtgag atatctttaa ccctgatcct ggcaatttcg
      601 gctatacgta acagggtgtt ataagcaatc cccagaaatg ccagattacg tatatcctgg
      661 cagcgatcgc tattttccat gagtgaacga acctggtcga aatcagtgcg ttcgaacgct
      721 agagcctgtt ttgcacgttc accggcatca acgttttctt ttcggatacg ccgcataacc
      781 agtgaaacag cattgctgtc acttggtcgt ggcagcccgg accgacgatg aagcatgttt
      841 agctggccca aatgttgctg gatagttt
//
