LOCUS       sequence-112543-         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GAL4-responsive UAS ""flp-On"" spaghetti monster GFP-HA reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Nern A, Pfeiffer BD, Rubin GM
  TITLE     Optimized tools for multicolor stochastic labeling reveal diverse 
            stereotyped cell arrangements in the fly visual system.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 May 11. pii: 201506763.
  PUBMED    25964354
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 May 11. pii: 201506763."
COMMENT     Sequence Label: pJFRC201-10XUAS-FRT>STOP>FRT-myr::smGFP-HA
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(7..40)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          136..201
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             331..351
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    623..757
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(660..679)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     714..733
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(769..802)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 agatctgaag ttcctatact ttctagagaa taggaacttc taacgtaagc tagctagagg
       61 atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag
      121 atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt
      181 ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg
      241 aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag
      301 gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc
      361 aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact
      421 cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt
      481 atggaaaaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
      541 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      601 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      661 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      721 gtccaaactc atcaatgtat cttatcatgt ctggatcgat ctggccggga agttcctata
      781 ctttctagag aataggaact tcctcgagaa tcaaaatggg caacaaatgc tgcagcaagc
      841 gacaggatca ggaactggca ctggcctatc ccactggggg ctacaagaaa tccgactaca
      901 cctttg
//
