LOCUS       sequence-112544-         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GAL4-responsive UAS ""flp-On"" spaghetti monster GFP-HA reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Nern A, Pfeiffer BD, Rubin GM
  TITLE     Optimized tools for multicolor stochastic labeling reveal diverse 
            stereotyped cell arrangements in the fly visual system.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 May 11. pii: 201506763.
  PUBMED    25964354
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 May 11. pii: 201506763."
COMMENT     Sequence Label: pJFRC201-10XUAS-FRT>STOP>FRT-myr::smGFP-HA
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    15..149
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(39..58)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     93..112
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(421..441)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          571..636
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(723..749)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(753..779)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(783..809)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cggccggcca gatcgatcca gacatgataa gatacattga tgagtttgga caaaccacaa
       61 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
      121 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
      181 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
      241 tggctgatta tgatctctag tcaaggcact atacatcaaa tatttttcca taattttctt
      301 gtatagcagt gcagcttttt cctttgtggt gtaaatagca aagcaagcaa gagttctatt
      361 actaaacaca gcatgactca aaaaacttag caattctgaa ggaaagtcct tggggtcttc
      421 tacctttctc ttcttttttg gaggagtaga atgttgagag tcagcagtag cctcatcatc
      481 actagatggc atttcttctg agcaaaacag gttttcctca ttaaaggcat tccaccactg
      541 ctcccattca tcagttccat aggttggaat ctaaaataca caaacaatta gaatcagtag
      601 tttaacacat tatacactta aaaattttat atttacctta gagctttaaa tctctgtagg
      661 tagtttgtcc aattatgtca caccacagaa gtaaggttcc ttcacaaaga tcctctagat
      721 taagcgtagt ccgggacatc gtacgggtaa ccggcatagt ccggtacgtc atacggatat
      781 ccagcgtaat ccggcacatc gtaggggtag ccacccttgt agagttcgtc catacccagg
      841 gtgattccag ccgcggtcac aaactccagc aggaccatgt gatcacgctt ttcgttcggg
      901 tcttt
//
