LOCUS       sequence-112547-         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mCherry fused between Cry2 and RGS4(del 1-33) to study cell 
            migration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   O'Neill PR, Gautam N
  TITLE     Subcellular optogenetic inhibition of G proteins generates signaling
            gradients and cell migration.
  JOURNAL   Mol Biol Cell. 2014 Aug 1;25(15):2305-14. doi: 
            10.1091/mbc.E14-04-0870. Epub 2014 Jun 11.
  PUBMED    24920824
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E14-04-0870"; journalName: "Mol Biol Cell"; date: 
            "2014-08-1- 1"; volume: "25"; issue: "15"; pages: "2305-14"
COMMENT     Sequence Label: Cry2 mCherry RGS4(del 1-33) in pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(804..821)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    810..921
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
       61 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
      121 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
      181 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
      241 gggtaccatg gaacatagtt cttcacacag caagaaggac aaagtagtaa cttgccagag
      301 ggtgagccaa gaagaagtca agaaatgggc tgaatcgctg gaaaacctga ttaaccatga
      361 atgtggactg gcagctttca aagctttcct gaagtcagaa tacagtgagg agaacattga
      421 cttctggatc agctgtgagg agtacaagaa aatcaaatca ccttctaaac taagtcccaa
      481 ggccaagaag atctacaatg agttcatctc tgtgcaggca acaaaagagg tgaacctgga
      541 ttcttgcacc agagaggaga caagccggaa catgttagag cccacgataa cctgttttga
      601 tgaagcccag aagaagattt tcaacctgat ggaaaaggat tcataccgtc gtttcctcaa
      661 gtctcgattc tatcttgacc tgaccaatcc ttccagctgc ggggcagaga agcagaaagg
      721 agccaagagt tctgcagact gtgcttccct ggtccctcag tgtgccttgt agtctagagg
      781 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
      841 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
      901 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc ggggggtggg
      961 gtggggcaga acacca
//
