LOCUS       sequence-112549-         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GAL4-responsive UAS "flp-On" spaghetti monster GFP-cMyc reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Nern A, Pfeiffer BD, Rubin GM
  TITLE     Optimized tools for multicolor stochastic labeling reveal diverse 
            stereotyped cell arrangements in the fly visual system.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 May 11. pii: 201506763.
  PUBMED    25964354
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 May 11. pii: 201506763."
COMMENT     Sequence Label: pJFRC203-10XUAS-FRT>STOP>FRT-myr::smGFP-cMyc
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    21..155
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(45..64)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     99..118
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(427..447)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          577..642
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(729..758)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(762..791)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(795..824)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tcgttacggc cggccagatc gatccagaca tgataagata cattgatgag tttggacaaa
       61 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
      121 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
      181 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
      241 gtggtatggc tgattatgat ctctagtcaa ggcactatac atcaaatatt tttccataat
      301 tttcttgtat agcagtgcag ctttttcctt tgtggtgtaa atagcaaagc aagcaagagt
      361 tctattacta aacacagcat gactcaaaaa acttagcaat tctgaaggaa agtccttggg
      421 gtcttctacc tttctcttct tttttggagg agtagaatgt tgagagtcag cagtagcctc
      481 atcatcacta gatggcattt cttctgagca aaacaggttt tcctcattaa aggcattcca
      541 ccactgctcc cattcatcag ttccataggt tggaatctaa aatacacaaa caattagaat
      601 cagtagttta acacattata cacttaaaaa ttttatattt accttagagc tttaaatctc
      661 tgtaggtagt ttgtccaatt atgtcacacc acagaagtaa ggttccttca caaagatcct
      721 ctagattaca gatcctcttc gctgataagt ttctgctccg ccaggtcctc ttcgctgatc
      781 agcttctgtt ccgccagatc ctcctcggag ataagtttct gttcagcgcc cttgtacagt
      841 tcgtccatgc ccaacgtgat gcccgcagcc gtgacgaact caagaagaac catgtggtca
      901 cgtttttcat tcgggtcctt gct
//
