LOCUS       sequence-112550-         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses intein-Cas9(S219) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Davis KM, Pattanayak V, Thompson DB, Zuris JA, Liu DR
  TITLE     Small molecule-triggered Cas9 protein with improved genome-editing 
            specificity.
  JOURNAL   Nat Chem Biol. 2015 May;11(5):316-8. doi: 10.1038/nchembio.1793. 
            Epub 2015 Apr 6.
  PUBMED    25848930
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1793"; journalName: "Nat Chem Biol"; date: 
            "2015-05"; volume: "11"; issue: "5"; pages: "316-8"
COMMENT     Sequence Label: pKMD106e - intein-Cas9(S219)
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..87)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(94..114)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gatgaccggt catcctgcag ccttgtcatc gtcatccttg taatcgatgt catgatcttt
       61 ataatcaccg tcatggtctt tgtagtcgct cgagactttc ctcttcttct tgggggatcc
      121 gtcaccccca agctgtgaca aatctatccg agtttcatat aatcccgtga tggattggtg
      181 aatcagtgtc gcgtctagca cctccttggt agaagtgtat cgtttgcgat ctatcgttgt
      241 gtcaaaatac ttgaatgcgg ctggagcgcc gaggttggta agagtaaaca aatggataat
      301 attttccgcc tgctcacgta tgggtttatc cctgtgcttg ttgtatgcgc ttaatacttt
      361 gtccagattg gcatcagcta ggatgactct cttactgaat tccgaaattt gctctatgat
      421 ttcgtcgaga taatgtttgt gctgctcaac aaaaagttgc ttctgttcgt tatcttcagg
      481 tgaacctttc aacttctcgt aatgggacgc taaatacagg aaattcacgt atttagacgg
      541 tagtgcgagt tcgttcccct tttgaagctc tccggcgcta gccaacatcc gttttcggcc
      601 attttctaac tcaaacagac tatactttgg tagtttaatt atgagatcct tttttacttc
      661 cttgtaacct ttcgcctcaa ggaagtcgat ggggttcttt tcaaaagacg agcgctccat
      721 aatcgttatc cccaataatt ctttgactga cttcagtttc ttggattttc ccttctcaac
      781 ttttgccact actaggacag aataggcaac tgtagggcta tcgaagccac cgtacttt
//
