LOCUS       sequence-112556-         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses intein-Cas9(S219-G521R) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Davis KM, Pattanayak V, Thompson DB, Zuris JA, Liu DR
  TITLE     Small molecule-triggered Cas9 protein with improved genome-editing 
            specificity.
  JOURNAL   Nat Chem Biol. 2015 May;11(5):316-8. doi: 10.1038/nchembio.1793. 
            Epub 2015 Apr 6.
  PUBMED    25848930
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1793"; journalName: "Nat Chem Biol"; date: 
            "2015-05"; volume: "11"; issue: "5"; pages: "316-8"
COMMENT     Sequence Label: pKMD111e - intein-Cas9(S219-G521R)
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(28..93)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(100..120)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggtgatgatg accggtcatc ctgcagcctt gtcatcgtca tccttgtaat cgatgtcatg
       61 atctttataa tcaccgtcat ggtctttgta gtcgctcgag actttcctct tcttcttggg
      121 ggatccgtca cccccaagct gtgacaaatc tatccgagtt tcatataatc ccgtgatgga
      181 ttggtgaatc agtgtcgcgt ctagcacctc cttggtagaa gtgtatcgtt tgcgatctat
      241 cgttgtgtca aaatacttga atgcggctgg agcgccgagg ttggtaagag taaacaaatg
      301 gataatattt tccgcctgct cacgtatggg tttatccctg tgcttgttgt atgcgcttaa
      361 tactttgtcc agattggcat cagctaggat gactctctta ctgaattccg aaatttgctc
      421 tatgatttcg tcgagataat gtttgtgctg ctcaacaaaa agttgcttct gttcgttatc
      481 ttcaggtgaa cctttcaact tctcgtaatg ggacgctaaa tacaggaaat tcacgtattt
      541 agacggtagt gcgagttcgt tccccttttg aagctctccg gcgctagcca acatccgttt
      601 tcggccattt tctaactcaa acagactata ctttggtagt ttaattatga gatccttttt
      661 tacttccttg taacctttcg cctcaaggaa gtcgatgggg ttcttttcaa aagacgagcg
      721 ctccataatc gttatcccca ataattcttt gactgacttc agtttcttgg attttccctt
      781 ctcaactttt gccactacta ggacagaata ggcaactgta gggctatcga agccaccgta
      841 cttt
//
