LOCUS       sequence-112563-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Tiam DH-PH domains fused to mYFP-PIF6, for optogenetic 
            recruitment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Levskaya A, Weiner OD, Lim WA, Voigt CA
  TITLE     Spatiotemporal control of cell signalling using a light-switchable 
            protein interaction.
  JOURNAL   Nature. 2009 Oct 15;461(7266):997-1001.
  PUBMED    19749742
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2009-10-15"; volume: "461(7266)"; pages: "997-1001"
COMMENT     Sequence Label: pAL188
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(9..28)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        134..344
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      203..338
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
       61 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
      121 accgacaatt gaggcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
      181 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
      241 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
      301 gctattccag aagtagtgag gaggcttttt tggaggccta ggctggtacc atgcgacagc
      361 tgtcggatgc ggataagctg cgcaaggtga tctgtgaact gctggagact gaacgcacct
      421 atgtgaaaga cttaaactgc ctcatggaga gatacctgaa gccccttcag aaggagacct
      481 ttctcaccca ggatgagctt gatgtacttt ttggaaattt aaccgaaatg gtggagtttc
      541 aagtcgagtt ccttaagact ctagaagatg gagtaagact ggtccctgac ttggaaaagc
      601 tggagaaggt tgaccagttc aagaaagtgc tcttctctct ggggggatcc ttcctgtact
      661 acgcggaccg cttcaagctc tacagtgcct tctgtgcgag ccacacaaaa gtccccaagg
      721 tcctggtgaa agccaagacg gacacagcct tcaaggcgtt cctcgatgcc cagaacccga
      781 ggcagcagca ctcatccacg ctggagtctt acctcatcaa gcccatccag agggtcctca
      841 agtacccgct tctgctcagg gagctgtttg cgctgaccga tgcggagagc gaggaacact
      901 accacctgga cgtgg
//
