LOCUS       sequence-112566-        4587 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter to measure miR-155 mediated differential 
            repression. Contains ELOVL6 3' UTR and mutated miR-155 sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Nam JW, Rissland OS, Koppstein D, Abreu-Goodger C, Jan CH, Agarwal 
            V, Yildirim MA, Rodriguez A, Bartel DP
  TITLE     Global analyses of the effect of different cellular contexts on 
            microRNA targeting.
  JOURNAL   Mol Cell. 2014 Mar 20;53(6):1031-43. doi: 
            10.1016/j.molcel.2014.02.013. Epub 2014 Mar 13.
  PUBMED    24631284
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.02.013"; journalName: "Mol Cell"; date: 
            "2014-03-20- 20"; volume: "53"; issue: "6"; pages: "1031-43"
COMMENT     Sequence Label: pIS1 mELOVL6
FEATURES             Location/Qualifiers
     source          1..4587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1466..1587
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1477..1496)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1531..1550
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        1720..1824
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1825..1893,1894..2685)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2043..2062)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2856..3444
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3345..3364
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3519..4270
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     intron          4340..4472
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     4455..4474
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        4517..4535
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(4545..4587,1..893)
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
ORIGIN
        1 taactggtcc gcagtggtgg gccagatgta aacaaatgaa tgttcttgat tcatttatta
       61 attattatga ttcagaaaaa catgcagaaa atgctgttat ttttttacat ggtaacgcgg
      121 cctcttctta tttatggcga catgttgtgc cacatattga gccagtagcg cggtgtatta
      181 taccagacct tattggtatg ggcaaatcag gcaaatctgg taatggttct tataggttac
      241 ttgatcatta caaatatctt actgcatggt ttgaacttct taatttacca aagaagatca
      301 tttttgtcgg ccatgattgg ggtgcttgtt tggcatttca ttatagctat gagcatcaag
      361 ataagatcaa agcaatagtt cacgctgaaa gtgtagtaga tgtgattgaa tcatgggatg
      421 aatggcctga tattgaagaa gatattgcgt tgatcaaatc tgaagaagga gaaaaaatgg
      481 ttttggagaa taacttcttc gtggaaacca tgttgccatc aaaaatcatg agaaagttag
      541 aaccagaaga atttgcagca tatcttgaac cattcaaaga gaaaggtgaa gttcgtcgtc
      601 caacattatc atggcctcgt gaaatcccgt tagtaaaagg tggtaaacct gacgttgtac
      661 aaattgttag gaattataat gcttatctac gtgcaagtga tgatttacca aaaatgttta
      721 ttgaatcgga cccaggattc ttttccaatg ctattgttga aggtgccaag aagtttccta
      781 atactgaatt tgtcaaagta aaaggtcttc atttttcgca agaagatgca cctgatgaaa
      841 tgggaaaata tatcaaatcg ttcgttgagc gagttctcaa aaatgaacaa taattctagg
      901 agctctatac cggtctcgat atcactacta gtgttctaga gctgtagacc ccatgagaaa
      961 agatgtctta atcatcctta tgaaaacaga tgtaaagaac tatatttcaa ctaacttcat
     1021 cttcactgca tagcctcagg ctagtgagtt tgccaaaacc aaagggggtg aatacttccc
     1081 caagattctt cctgggagga tggaaacagt gcagcccagg tcccatgggg gcagctccat
     1141 cccagagcat ttctgatagt tgaactgtaa tttctactct taagtgagat atgaaacgta
     1201 atccttttgt tcagttgccc cgggcttttg aacagaagag taaatacaga attgaaaaag
     1261 ataaacactc aaccaaacaa tgtgaaaacg ggttctgtag tatttgtaaa aaggcccggc
     1321 ccaggaccac tgtgagctgg aaaagggaga aaggcagtgg gaaaagaggt gagccgaaga
     1381 tcaattcgac agacagacgg tgtgtatgcc cctccctgtt tgacttcaca cacactcata
     1441 acgcggccgc ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact
     1501 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     1561 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     1621 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa
     1681 atcgataagg atccaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     1741 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     1801 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     1861 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     1921 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     1981 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     2041 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     2101 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     2161 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     2221 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     2281 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     2341 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     2401 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     2461 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     2521 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     2581 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     2641 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     2701 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     2761 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     2821 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2881 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2941 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     3001 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     3061 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     3121 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     3181 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     3241 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     3301 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     3361 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     3421 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3481 ttttgctggc cttttgctca catggctcga cagatctaaa tgagtcttcg gacctcgcgg
     3541 gggccgctta agcggtggtt agggtttgtc tgacgcgggg ggagggggaa ggaacgaaac
     3601 actctcattc ggaggcggct cggggtttgg tcttggtggc cacgggcacg cagaagagcg
     3661 ccgcgatcct cttaagcacc cccccgccct ccgtggaggc gggggtttgg tcggcgggtg
     3721 gtaactggcg ggccgctgac tcgggcgggt cgcgcgcccc agagtgtgac cttttcggtc
     3781 tgctcgcaga cccccgggcg gcgccgccgc ggcggcgacg ggctcgctgg gtcctaggct
     3841 ccatggggac cgtatacgtg gacaggctct ggagcatccg cacgactgcg gtgatattac
     3901 cggagacctt ctgcgggacg agccgggtca cgcggctgac gcggagcgtc cgttgggcga
     3961 caaacaccag gacggggcac aggtacacta tcttgtcacc cggaggcgcg agggactgca
     4021 ggagcttcag ggagtggcgc agctgcttca tccccgtggc ccgttgctcg cgtttgctgg
     4081 cggtgtcccc ggaagaaata tatttgcatg tctttagttc tatgatgaca caaaccccgc
     4141 ccagcgtctt gtcattggcg aattcgaaca cgcagatgca gtcggggcgg cgcggtccca
     4201 ggtccacttc gcatattaag gtgacgcgtg tggcctcgaa caccgagcga ccctgcagcg
     4261 acccgcttaa aagcttgatt cttctgacac aacagtctcg aacttaagct gcagaagttg
     4321 gtcgtgaggc actgggcagg taagtatcaa ggttacaaga caggtttaag gagaccaata
     4381 gaaactgggc ttgtcgagac agagaagact cttgcgtttc tgataggcac ctattggtct
     4441 tactgacatc cactttgcct ttctctccac aggtgtccac tcccagttca attacagctc
     4501 ttaaggctag agtacttaat acgactcact ataggctagc caccatgact tcgaaagttt
     4561 atgatccaga acaaaggaaa cggatga
//
