LOCUS       sequence-112567-         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GAL4-responsive UAS "flp-On" spaghetti monster GFP-V5 reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Nern A, Pfeiffer BD, Rubin GM
  TITLE     Optimized tools for multicolor stochastic labeling reveal diverse 
            stereotyped cell arrangements in the fly visual system.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 May 11. pii: 201506763.
  PUBMED    25964354
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 May 11. pii: 201506763."
COMMENT     Sequence Label: pJFRC206-10XUAS-FRT>STOP>FRT-myr::smGFP-V5
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(8..41)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          137..202
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             332..352
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    624..758
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(661..680)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     715..734
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(770..803)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 tagatctgaa gttcctatac tttctagaga ataggaactt ctaacgtaag ctagctagag
       61 gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga
      121 gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat
      181 tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg
      241 gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga
      301 ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc
      361 caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac
      421 tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat
      481 tatggaaaaa tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat
      541 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
      601 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
      661 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
      721 tgtccaaact catcaatgta tcttatcatg tctggatcga tctggccggg aagttcctat
      781 actttctaga gaataggaac ttcctcgaga atcaaaatgg gcaacaaatg ctgcagcaag
      841 cgacaggatc aggaactggc actggcctat cccactgggg gctacaagaa atccgactac
      901 acctttggcc agacgcacat caacagcagc ggcggcggca ctggcg
//
