LOCUS       sequence-112570-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GAL4-responsive UAS "flp-On" spaghetti monster GFP-V5 reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Nern A, Pfeiffer BD, Rubin GM
  TITLE     Optimized tools for multicolor stochastic labeling reveal diverse 
            stereotyped cell arrangements in the fly visual system.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 May 11. pii: 201506763.
  PUBMED    25964354
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 May 11. pii: 201506763."
COMMENT     Sequence Label: pJFRC206-10XUAS-FRT>STOP>FRT-myr::smGFP-V5
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    17..151
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(41..60)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     95..114
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(423..443)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          573..638
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(725..766)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(776..817)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(827..868)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 tacggccggc cagatcgatc cagacatgat aagatacatt gatgagtttg gacaaaccac
       61 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
      121 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
      181 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
      241 tatggctgat tatgatctct agtcaaggca ctatacatca aatatttttc cataattttc
      301 ttgtatagca gtgcagcttt ttcctttgtg gtgtaaatag caaagcaagc aagagttcta
      361 ttactaaaca cagcatgact caaaaaactt agcaattctg aaggaaagtc cttggggtct
      421 tctacctttc tcttcttttt tggaggagta gaatgttgag agtcagcagt agcctcatca
      481 tcactagatg gcatttcttc tgagcaaaac aggttttcct cattaaaggc attccaccac
      541 tgctcccatt catcagttcc ataggttgga atctaaaata cacaaacaat tagaatcagt
      601 agtttaacac attatacact taaaaatttt atatttacct tagagcttta aatctctgta
      661 ggtagtttgt ccaattatgt cacaccacag aagtaaggtt ccttcacaaa gatcctctag
      721 attaggtact atccagtccc agcaacgggt tcgggatggg cttgccctgc tgttgcgtac
      781 tgtcgaggcc caacagtgga tttggtattg gctttccttg ttgctgagtg ctgtcgaggc
      841 ccagcaaggg atttggtatg ggcttgcc
//
