LOCUS       sequence-112571-         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GAL4-responsive UAS "flp-On" spaghetti monster GFP-FLAG reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Nern A, Pfeiffer BD, Rubin GM
  TITLE     Optimized tools for multicolor stochastic labeling reveal diverse 
            stereotyped cell arrangements in the fly visual system.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 May 11. pii: 201506763.
  PUBMED    25964354
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 May 11. pii: 201506763."
COMMENT     Sequence Label: pJFRC208-10XUAS-FRT>STOP>FRT-myr::smGFP-FLAG
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(16..49)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          145..210
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             340..360
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    632..766
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(669..688)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     723..742
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(778..811)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 actctgaata gatctgaagt tcctatactt tctagagaat aggaacttct aacgtaagct
       61 agctagagga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta
      121 cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa
      181 ctactgattc taattgtttg tgtattttag attccaacct atggaactga tgaatgggag
      241 cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt
      301 gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta
      361 gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt
      421 aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac
      481 aagaaaatta tggaaaaata tttgatgtat agtgccttga ctagagatca taatcagcca
      541 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
      601 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
      661 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
      721 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcgatc tggccgggaa
      781 gttcctatac tttctagaga ataggaactt cctcgagaat caaaatgggc aacaaatgct
      841 gcagcaagcg acaggatcag gaactggcac tggcctatcc cactgggggc tacaagaaat
      901 ccgactacac ctttggccag acgcacatca ccagcagcgg cggcggcatc
//
