LOCUS       sequence-112579-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence
            (TaU3 promoter), Bar resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pBUN421
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(19..66)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     RBS             146..154
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 tatggaggcc tgagctcact tcttcttctt cgcctgcccc gccttcttcg tcgccgctgg
       61 ccgcttgtcg cccccgagct gagacaggtc gatgcgcgtc tcgtagaggc cggtaatcga
      121 ctggtggatg agggtcgcgt ccaggacctc cttagtgctt gtgtacctct tgcgatcgat
      181 agttgtgtcg aagtacttga aagcagcagg ggcgccgagg ttcgtcaggg tgaagagatg
      241 aatgatattc tcagcctgct ccctgattgg cttgtcgcgg tgcttgttgt acgcggagag
      301 gaccttatcc agattcgcgt cggccaggat cacgcgcttg gagaactcgg aaatctgctc
      361 aatgatctcg tcgaggtaat gcttgtgctg ctcgacgaac agctgcttct gctcgttgtc
      421 ctcggggctg cccttgagct tctcgtagtg ggaggccagg tagaggaagt tcacatactt
      481 ggacggcaga gccagctcgt tccccttctg cagctcgcca gcggaagcca gcatccgctt
      541 cctgccgttc tccagctcga agagtgagta cttggggagc ttaatgatca ggtccttctt
      601 cacctccttg tagcccttcg cctccaggaa atcgatcggg ttcttctcga agctggagcg
      661 ctccataatc gtgatcccca gcagctcctt cacgctcttg agcttcttgg acttgccctt
      721 ctcaaccttc gccacaacca ggaccgagta ggccacagtg gggctgtcga acccgccgta
      781 cttcttcgga tcccagtcct tcttgcgggc gatgagcttg tcgctgttcc gcttaggcag
      841 aattgactcc
//
