LOCUS       sequence-112582-        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  First-generation packaging plasmid. This plasmid contains HIV 
            accessory genes. Please reach out to your biosafety officer for 
            guidance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  TITLE     Trono Lab Packaging and Envelope Plasmids
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCMV delta R8.2
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             127..153
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
ORIGIN
        1 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
       61 cattcgatta gtgaacggat ccttggcact tatctgggac gatctgcgga gcctgtgcct
      121 cttcagctac caccgcttga gagacttact cttgattgta acgaggattg tggaacttct
      181 gggacgcagg gggtgggaag ccctcaaata ttggtggaat ctcctacaat attggagtca
      241 ggagctaaag aatagtgctg ttagcttgct caatgccaca gccatagcag tagctgaggg
      301 gacagatagg gttatagaag tagtacaagg agcttgtaga gctattcgcc acatacctag
      361 aagaataaga cagggcttgg aaaggatttt gctataagat gggtggcaag tggtcaaaaa
      421 gtagtgtgat tggatggcct actgtaaggg aaagaatgag acgagctgag ccagcagcag
      481 atggggtggg agcagtatct cgagacctag aaaaacatgg agcaatcaca agtagcaata
      541 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
      601 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
      661 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
      721 atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca
      781 caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag
      841 ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg
      901 tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc
      961 gcctagcatt tcatcacatg gcccgagagc tgcatccgga
//
