LOCUS       sequence-112590-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ubiquitous expression of a ""flp-Out"" Drosophila codon-optimized 
            GAL80 gene cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Nern A, Pfeiffer BD, Rubin GM
  TITLE     Optimized tools for multicolor stochastic labeling reveal diverse 
            stereotyped cell arrangements in the fly visual system.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 May 11. pii: 201506763.
  PUBMED    25964354
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 May 11. pii: 201506763."
COMMENT     Sequence Label: tubP-FRT>GAL80-6-FRT>
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(291..324)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          353..419
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
ORIGIN
        1 aattcggtta ccatgatact tcgacgcata actgtagatt ttggatagaa ttaaagagaa
       61 aatggcgaga gagtaaaatt ccggcgtcgg caaagtagag caaaaaaatc agtataccat
      121 ttagctacct ctctcactcg cacgcagtgc cggctcaagt tgggcgcggc tctgcaatta
      181 tcgattttct tggggtgtgt aactaatcat ccgttttccc ttcctcctca tccacagcgt
      241 gaagaattcg tacatctaga ataaacggcc ggccagatct ggtaccatct gaagttccta
      301 tactttctag agaataggaa cttctaacgt aagctagcta gaccggtaaa aggtaggttc
      361 aaccactgat gcctaggcac accgaaacga ctaaccctaa ttcttatcct ttacttcagg
      421 ctagcaatca aaatggatta caacaaaagg agtagtgtga gtacggtgcc gaatgctgct
      481 cccattcgcg tgggcttcgt gggattgaac gcggctaagg gttgggccat taaaacgcat
      541 tatccagcca tactgcagct gagctcccag ttccaaataa cagcgcttta ttcccccaag
      601 atcgagacgt ccattgcgac aattcagagg ctgaagttgt ccaacgccac agctttccca
      661 acactcgaga gcttcgcctc gagctccacg atcgatatga ttgtgatcgc aatccaggtg
      721 gcttcccact acgaggtggt aatgcccctg ctcgagtttt cgaagaacaa tcctaatctg
      781 aagtatttgt ttgtggaatg ggcactggcc tgctcgctcg atcaggcgga tc
//
