LOCUS       sequence-112595-       10892 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Const. Halotag-GFP-Nuf2 + mCherry-DHFR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10892)
  AUTHORS   Ballister ER, Aonbangkhen C, Mayo AM, Lampson MA, Chenoweth DM
  TITLE     Localized light-induced protein dimerization in living cells using a
            photocaged dimerizer.
  JOURNAL   Nat Commun. 2014 Nov 17;5:5475. doi: 10.1038/ncomms6475.
  PUBMED    25400104
REFERENCE   2  (bases 1 to 10892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-11-17"; volume: "5:5475. doi"; pages: " 
            10.1038/ncomms6475"
COMMENT     Sequence Label: pERB215
FEATURES             Location/Qualifiers
     source          1..10892
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    52..100
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    312..345
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             350..949
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(350..369)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     846..866
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1085..1102)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1091..1202
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        1347..1650
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1652..1929
                     /label=chicken beta-actin promoter
     intron          1930..2946
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2870..2892
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2954..2973
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      3031..3040
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3037..3927
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     regulatory      3934..3943
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3940..3942,3943..3945,3946..4656)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3940..3942,3943..3945,3946..4656)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3985..4006)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4246..4265)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4593..4614
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6128..6701
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6151..6703
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6295..6312)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6522..6541
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6703..7410
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6848..6866)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7116..7135
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             7429..7902
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="ISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHT
                     WESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP
                     KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     CDS             7903..7932
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(8039..8058)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8104..8159
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8158..8177)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     protein_bind    8520..8553
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    8566..8687
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8603..8622)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8657..8676
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8706..8726)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8706..8724)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8745..8761)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8745..8761)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8758..8780)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8769..8785
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8793..8799,8800..8817,8818..8823))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8838..8859
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8976..8993)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9147..9735)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9227..9246)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9906..10697,10698..10766))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10529..10548
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10767..10871)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aagcttaata aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
       61 aaagacagaa taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
      121 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
      181 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
      241 gtcggggcgg caggccctgc catagccact ggccccgtgg gttagggacg gggtccccca
      301 ttcgagaata aataacttcg tataaagtat cctatacgaa gttatcacca tgaccgagta
      361 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc
      421 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga
      481 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
      541 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
      601 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
      661 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
      721 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
      781 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
      841 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
      901 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca
      961 cgacccgcag cgcccgaccg aaaggagcgc acgaccccat ggctccgacc gaagccgacc
     1021 cgggcggccc cgccgacccc gcacccgccc ccgaggccca ccgactctag agctcgctga
     1081 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     1141 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     1201 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     1261 ggggaggatt gggaagacaa tagcactagt tattaatagt aatcaattac ggggtcatta
     1321 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     1381 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     1441 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     1501 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     1561 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1621 atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact
     1681 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt
     1741 tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc
     1801 gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc
     1861 cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg
     1921 cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg
     1981 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc
     2041 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg
     2101 ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg
     2161 ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc
     2221 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc
     2281 gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg
     2341 cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc
     2401 cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt
     2461 acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg
     2521 ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg
     2581 agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc
     2641 gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc
     2701 cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg
     2761 ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg
     2821 ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc
     2881 tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc
     2941 ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattg
     3001 ctcgaccacc ggcgcgctgc agtacggcgc gccaccatgg cagaaatcgg tactggcttt
     3061 ccattcgacc cccattatgt ggaagtcctg ggcgagcgca tgcactacgt cgatgttggt
     3121 ccgcgcgatg gcacccctgt gctgttcctg cacggtaacc cgacctcctc ctacgtgtgg
     3181 cgcaacatca tcccgcatgt tgcaccgacc catcgctgca ttgctccaga cctgatcggt
     3241 atgggcaaat ccgacaaacc agacctgggt tatttcttcg acgaccacgt ccgcttcatg
     3301 gatgccttca tcgaagccct gggtctggaa gaggtcgtcc tggtcattca cgactggggc
     3361 tccgctctgg gtttccactg ggccaagcgc aatccagagc gcgtcaaagg tattgcattt
     3421 atggagttca tccgccctat cccgacctgg gacgaatggc cagaatttgc ccgcgagacc
     3481 ttccaggcct tccgcaccac cgacgtcggc cgcaagctga tcatcgatca gaacgttttt
     3541 atcgagggta cgctgccgat gggtgtcgtc cgcccgctga ctgaagtcga gatggaccat
     3601 taccgcgagc cgttcctgaa tcctgttgac cgcgagccac tgtggcgctt cccaaacgag
     3661 ctgccaatcg ccggtgagcc agcgaacatc gtcgcgctgg tcgaagaata catggactgg
     3721 ctgcaccagt cccctgtccc gaagctgctg ttctggggca ccccaggcgt tctgatccca
     3781 ccggccgaag ccgctcgcct ggccaaaagc ctgcctaact gcaaggctgt ggacatcggc
     3841 ccgggtctga atctgctgca agaagacaac ccggacctga tcggcagcga gatcgcgcgc
     3901 tggctgtcga cgctcgagat ttccggttac gtagccacca tggtgagcaa gggcgaggag
     3961 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     4021 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     4081 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     4141 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     4201 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     4261 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     4321 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     4381 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     4441 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     4501 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     4561 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     4621 gccgggatca ctctcggcat ggacgagctg tacaagggtg gtgaatccgg tgatgcatcg
     4681 cgagctcaag cttcgaattc aggccggcca accatggaaa ctttgtcttt ccccagatat
     4741 aatgtagctg agattgtgat tcatattcgc aataagatct taacaggagc tgatggtaaa
     4801 aacctcacca agaatgatct ttatccaaat ccaaagcctg aagtcttgca catgatctac
     4861 atgagagcct tacaaatagt atatggaatt cgactggaac atttttacat gatgccagtg
     4921 aactctgaag tcatgtatcc acatttaatg gaaggcttct taccattcag caatttagtt
     4981 actcatctgg actcattttt gcctatctgc cgggtgaatg actttgagac tgctgatatt
     5041 ctatgtccaa aagcaaaacg gacaagtcgg tttttaagtg gcattatcaa ctttattcac
     5101 ttcagagaag catgccgtga aacgtatatg gaatttcttt ggcaatataa atcctctgcg
     5161 gacaaaatgc aacagttaaa cgccgcacac caggaggcat taatgaaact ggagagactt
     5221 gattctgttc cagttgaaga gcaagaagag ttcaagcagc tttcagatgg tattcaggag
     5281 ctacaacaat cactaaatca ggattttcat caaaaaacga tagtgctgca agagggaaat
     5341 tcccaaaaga agtcaaatat ttcagagaaa accaagcgtt tgaatgaact aaaattgttg
     5401 gtggtttctt tgaaagaaat acaagagagt ttgaaaacaa aaattgtgga ttctccagag
     5461 aagttaaaga attataaaga aaaaatgaaa gatacggtcc agaagcttaa aaatgccaga
     5521 caagaagtgg tggagaaata tgaaatctat ggagactcag ttgactgcct gccttcatgt
     5581 cagttggaag tgcagttata tcaaaagaaa atacaggacc tttcagataa tagggaaaaa
     5641 ttagccagta tcttaaagga gagcctgaac ttggaggacc aaattgagag tgatgagtca
     5701 gaactgaaga aattgaagac tgaagaaaat tcgttcaaaa gactgatgat tgtgaagaag
     5761 gaaaaacttg ccacagcaca attcaaaata aataagaagc atgaagatgt taagcaatac
     5821 aaacgcacag taattgagga ttgcaataaa gttcaagaaa aaagaggtgc tgtctatgaa
     5881 cgagtaacca caattaatca agaaatccaa aaaattaaac ttggaattca acaactaaaa
     5941 gatgctgctg aaagggagaa actgaagtcc caggaaatat ttctaaactt gaaaactgct
     6001 ttggagaaat accacgacgg tattgaaaag gcagcagagg actcctatgc taagatagat
     6061 gagaagacag ctgaactgaa gaggaagatg ttcaaaatgt ccggatgacg ataagcttaa
     6121 ggatccgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
     6181 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     6241 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     6301 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     6361 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     6421 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     6481 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     6541 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     6601 ctcggtacac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
     6661 accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggtgag caagggcgag
     6721 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     6781 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     6841 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     6901 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     6961 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     7021 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     7081 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     7141 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     7201 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     7261 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
     7321 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     7381 tccaccggcg gcatggacga gctgtataag gccggcctgg agtctagaat cagtctgatt
     7441 gcggcgttag cggtagatcg cgttatcggc atggaaaacg ccatgccgtg gaacctgcct
     7501 gccgatctcg cctggtttaa acgcaacacc ttaaataaac ccgtgattat gggccgccat
     7561 acctgggaat caatcggtcg tccgttgcca ggacgcaaaa atattatcct cagcagtcaa
     7621 ccgggtacgg acgatcgcgt aacgtgggtg aagtcggtgg atgaagccat cgcggcgtgt
     7681 ggtgacgtac cagaaatcat ggtgattggc ggcggtcgcg tttatgaaca gttcttgcca
     7741 aaagcgcaaa aactgtatct gacgcatatc gacgcagaag tggaaggcga cacccatttc
     7801 ccggattacg agccggatga ctgggaatcg gtattcagcg aattccacga tgctgatgcg
     7861 cagaactctc acagctattg ctttgagatt ctggagcggc gggaacaaaa actcatctca
     7921 gaagaggatc tttaattaag cgctcgaggc ggccgcaatt cactcctcag gtgcaggctg
     7981 cctatcagaa ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc
     8041 tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg
     8101 gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac
     8161 tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga
     8221 gtttggcaac atatgccata tgctggctgc catgaacaaa ggtggctata aagaggtcat
     8281 cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga
     8341 ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt
     8401 tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct
     8461 gtccctcttc tcttatgaag atccctcgac ctgagcgtcg agctagcata tggatccata
     8521 taacttcgta taatgtatgc tatacgaagt tattacgata ccgtcaactt gtttattgca
     8581 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     8641 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaggt acccagcttt
     8701 tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct
     8761 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     8821 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     8881 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     8941 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     9001 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     9061 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     9121 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     9181 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     9241 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     9301 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     9361 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     9421 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     9481 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     9541 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     9601 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     9661 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     9721 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     9781 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     9841 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     9901 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     9961 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    10021 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    10081 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    10141 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    10201 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    10261 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    10321 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    10381 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    10441 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    10501 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    10561 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    10621 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    10681 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    10741 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    10801 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    10861 ggggttccgc gcacatttcc ccgaaaagtg cc
//
