LOCUS       sequence-112599-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a polyspecific aminoacyl-tRNA synthetase and four copies of 
            amber suppression tRNAs for unnatural amino acid incorporation in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Chen ZJ, Ren W, Wright QE, Ai HW
  TITLE     Genetically encoded fluorescent probe for the selective detection of
            peroxynitrite.
  JOURNAL   J Am Chem Soc. 2013 Oct 9;135(40):14940-3. doi: 10.1021/ja408011q. 
            Epub 2013 Sep 26.
  PUBMED    24059533
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja408011q";
            journalName: "J Am Chem Soc"; date: "2013-10-9- 9"; volume: "135"; 
            issue: "40"; pages: "14940-3"
COMMENT     Sequence Label: pMAH-POLY
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        221..239
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     221..238
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     252..271
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      304..313
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg
       61 accgatccag cctccgcggc cgggaacggt gcattggaac gcggattccc cgtgccaaga
      121 gtgacgtaag taccgcctat agagtctata ggcccacccc cttggcttcg ttagaacgcg
      181 gctacaatta atacataacc ttatgtatca tacacatacg atttaggtga cactatagaa
      241 taacatccac tttgcctttc tctccacagg tgtccactcc caggtccaac tgcacggaag
      301 cttgccacca tggcaagcag taacttgatt aaacaattgc aagagcgggg gctggtagcc
      361 caggtgacgg acgaggaagc gctagcagag cgactggcgc aaggcccgat cgcactcatt
      421 tgtggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc attgttatgc
      481 ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg cgcgacgggt
      541 ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacaccga agaaactgtt
      601 caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt cgactgtgga
      661 gaaaactctg ctatcgcggc caataattat gactggttcg gcaatatgaa tgtgctgacc
      721 ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa agaagcggtt
      781 aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc ctacaacctg
      841 ctgcagggtt attcgatggc c
//
