LOCUS       sequence-11260-e        3414 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-TIE1
FEATURES             Location/Qualifiers
     source          1..3414
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(538..549)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 atggtctggc gggtgccccc tttcttgctc cccatcctct tcttggcttc tcatgtgggc
       61 gcggcggtgg acctgacgct gctggccaac ctgcggctca cggaccccca gcgcttcttc
      121 ctgacttgcg tgtctgggga ggccggggcg gggaggggct cggacgcctg gggcccgccc
      181 ctgctgctgg agaaggacga ccgtatcgtg cgcaccccgc ccgggccacc cctgcgcctg
      241 gcgcgcaacg gttcgcacca ggtcacgctt cgcggcttct ccaagccctc ggacctcgtg
      301 ggcgtcttct cctgcgtggg cggtgctggg gcgcggcgca cgcgcgtcat ctacgtgcac
      361 aacagccctg gagcccacct gcttccagac aaggtcacac acactgtgaa caaaggtgac
      421 accgctgtac tttctgcacg tgtgcacaag gagaagcaga cagacgtgat ctggaagagc
      481 aacggatcct acttctacac cctggactgg catgaagccc aggatgggcg gttcctgctg
      541 cagctcccaa atgtgcagcc accatcgagc ggcatctaca gtgccactta cctggaagcc
      601 agccccctgg gcagcgcctt ctttcggctc atcgtgcggg gttgtggggc tgggcgctgg
      661 gggccaggct gtaccaagga gtgcccaggt tgcctacatg gaggtgtctg ccacgaccat
      721 gacggcgaat gtgtatgccc ccctggcttc actggcaccc gctgtgaaca ggcctgcaga
      781 gagggccgtt ttgggcagag ctgccaggag cagtgcccag gcatatcagg ctgccggggc
      841 ctcaccttct gcctcccaga cccctatggc tgctcttgtg gatctggctg gagaggaagc
      901 cagtgccaag aagcttgtgc ccctggtcat tttggggctg attgccgact ccagtgccag
      961 tgtcagaatg gtggcacttg tgaccggttc agtggttgtg tctgcccctc tgggtggcat
     1021 ggagtgcact gtgagaagtc agaccggatc ccccagatcc tcaacatggc ctcagaactg
     1081 gagttcaact tagagacgat gccccggatc aactgtgcag ctgcagggaa ccccttcccc
     1141 gtgcggggca gcatagagct acgcaagcca gacggcactg tgctcctgtc caccaaggcc
     1201 attgtggagc cagagaagac cacagctgag ttcgaggtgc cccgcttggt tcttgcggac
     1261 agtgggttct gggagtgccg tgtgtccaca tctggcggcc aagacagccg gcgcttcaag
     1321 gtcaatgtga aagtgccccc cgtgcccctg gctgcacctc ggctcctgac caagcagagc
     1381 cgccagcttg tggtctcccc gctggtctcg ttctctgggg atggacccat ctccactgtc
     1441 cgcctgcact accggcccca ggacagtacc atggactggt cgaccattgt ggtggacccc
     1501 agtgagaacg tgacgttaat gaacctgagg ccaaagacag gatacagtgt tcgtgtgcag
     1561 ctgagccggc caggggaagg aggagagggg gcctgggggc ctcccaccct catgaccaca
     1621 gactgtcctg agcctttgtt gcagccgtgg ttggagggct ggcatgtgga aggcactgac
     1681 cggctgcgag tgagctggtc cttgcccttg gtgcccgggc cactggtggg cgacggtttc
     1741 ctgctgcgcc tgtgggacgg gacacggggg caggagcggc gggagaacgt ctcatccccc
     1801 caggcccgca ctgccctcct gacgggactc acgcctggca cccactacca gctggatgtg
     1861 cagctctacc actgcaccct cctgggcccg gcctcgcccc ctgcacacgt gcttctgccc
     1921 cccagtgggc ctccagcccc ccgacacctc cacgcccagg ccctctcaga ctccgagatc
     1981 cagctgacat ggaagcaccc ggaggctctg cctgggccaa tatccaagta cgttgtggag
     2041 gtgcaggtgg ctgggggtgc aggagaccca ctgtggatag acgtggacag gcctgaggag
     2101 acaagcacca tcatccgtgg cctcaacgcc agcacgcgct acctcttccg catgcgggcc
     2161 agcattcagg ggctcgggga ctggagcaac acagtagaag agtccaccct gggcaacggg
     2221 ctgcaggctg agggcccagt ccaagagagc cgggcagctg aagagggcct ggatcagcag
     2281 ctgatcctgg cggtggtggg ctccgtgtct gccacctgcc tcaccatcct ggccgccctt
     2341 ttaaccctgg tgtgcatccg cagaagctgc ctgcatcgga gacgcacctt cacctaccag
     2401 tcaggctcgg gcgaggagac catcctgcag ttcagctcag ggaccttgac acttacccgg
     2461 cggccaaaac tgcagcccga gcccctgagc tacccagtgc tagagtggga ggacatcacc
     2521 tttgaggacc tcatcgggga ggggaacttc ggccaggtca tccgggccat gatcaagaag
     2581 gacgggctga agatgaacgc agccatcaaa atgctgaaag agtatgcctc tgaaaatgac
     2641 catcgtgact ttgcgggaga actggaagtt ctgtgcaaat tggggcatca ccccaacatc
     2701 atcaacctcc tgggggcctg taagaaccga ggttacttgt atatcgctat tgaatatgcc
     2761 ccctacggga acctgctaga ttttctgcgg aaaagccggg tcctagagac tgacccagct
     2821 tttgctcgag agcatgggac agcctctacc cttagctccc ggcagctgct gcgtttcgcc
     2881 agtgatgcgg ccaatggcat gcagtacctg agtgagaagc agttcatcca cagggacctg
     2941 gctgcccgga atgtgctggt cggagagaac ctagcctcca agattgcaga cttcggcctt
     3001 tctcggggag aggaggttta tgtgaagaag acgatggggc gtctccctgt gcgctggatg
     3061 gccattgagt ccctgaacta cagtgtctat accaccaaga gtgatgtctg gtcctttgga
     3121 gtccttcttt gggagatagt gagccttgga ggtacaccct actgtggcat gacctgtgcc
     3181 gagctctatg aaaagctgcc ccagggctac cgcatggagc agcctcgaaa ctgtgacgat
     3241 gaagtgtacg agctgatgcg tcagtgctgg cgggaccgtc cctatgagcg accccccttt
     3301 gcccagattg cgctacagct aggccgcatg ctggaagcca ggaaggccta tgtgaacatg
     3361 tcgctgtttg agaacttcac ttacgcgggc attgatgcca cagctgagga ggcc
//
