LOCUS       sequence-112602-         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Flag-tagged Med25-VWA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Sela D, Chen L, Martin-Brown S, Washburn MP, Florens L, Conaway JW, 
            Conaway RC
  TITLE     Endoplasmic reticulum stress-responsive transcription factor 
            ATF6alpha directs recruitment of the Mediator of RNA polymerase II 
            transcription and multiple histone acetyltransferase complexes.
  JOURNAL   J Biol Chem. 2012 Jun 29;287(27):23035-45. doi: 
            10.1074/jbc.M112.369504. Epub 2012 May 10.
  PUBMED    22577136
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.369504"; journalName: "J Biol Chem"; date: 
            "2012-06-29- 29"; volume: "287"; issue: "27"; pages: "23035-45"
COMMENT     Sequence Label: pcDNA5-Flag-Med25-VWA
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3..17
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     primer_bind     complement(765..782)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 aggacgatga tgacaagctc gagatggtcc ccgggtccga gggcccggcc cgcgccggga
       61 gcgtggtggc cgacgtggtg tttgtgattg agggtacggc caacctggga ccctacttcg
      121 aggggctccg caagcactac ctgctcccgg ccatcgagta ttttaatggt ggtcctcctg
      181 ctgagacgga cttcggggga gactatgggg ggacccagta cagcctcgtg gtgttcaaca
      241 cagtggactg cgctcccgag tcctacgtac aatgtcacgc tcccaccagc agcgcctatg
      301 agtttgtcac ctggctcgat ggcattaagt tcatgggcgg gggtggtgag agctgcagcc
      361 tcatcgcgga aggactcagc acagccttgc agctgtttga tgacttcaag aagatgcgcg
      421 agcagattgg ccagacgcac cgggtctgcc tcctcatctg caactcaccc ccatacttgt
      481 tgcctgctgt tgagagcacc acgtactctg gatgcacaac tgagaatctt gtgcagcaga
      541 ttggggagcg ggggatccac ttctccattg tgtctccccg gaagctgcct gcgcttcggc
      601 ttctgtttga gaaggcagcc cccccggcct tgctggagcc gctgcagcct ccgacagatg
      661 tgagccagga cccgaggcac atggtgctgg ttcggggact cgtgctgcct gtttaggcgg
      721 ccgctcgatc gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc
      781 tagttgccag ccatctgttg tttgcc
//
