LOCUS       sequence-112605-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bicistronic expression of mRFP-Utrophin and Neo resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  TITLE     Piel Lab Plasmids
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pIRESneo3+mRFP-Utr_CH
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(143..162)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(144..162)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(207..410)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(236..256)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        411..790
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tcgatctcga actcgtggcc gttcacggag ccctccatgc gcaccttgaa gcgcatgaac
       61 tccttgatga cgtcctcgga ggaggccatg gtgctagcta ggccgcagat atcgatccga
      121 gctcggtacc aagcttgggt ctccctatag tgagtcgtat taatttcgat aagccagtaa
      181 gcagtgggtt ctctagttag ccagagagct ctgcttatat agacctccca ccgtacacgc
      241 ctaccgccca tttgcgtcaa tggggcggag ttgttacgac attttggaaa gtcccgttga
      301 ttttggtgcc aaaacaaact cccattgacg tcaatggggt ggagacttgg aaatccccgt
      361 gagtcaaacc gctatccacg cccattgatg tactgccaaa accgcatcac catggtaata
      421 gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg
      481 gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata
      541 tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt
      601 caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg
      661 cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgcggaac
      721 tccatatatg ggctatgaac taatgacccc gtaattgatt actattaata actagtcaat
      781 aatcaatgtc aacgcgtata tctggcccgt acatcgcgaa gcagcgcaaa acgcctaacc
      841 ctaagcag
//
