LOCUS       sequence-112608-        1009 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bicistronic expression of Centrin-GFP fusion and Puro resistance 
            gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1009)
  TITLE     Piel Lab Plasmids
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pIRES Centrin1 GFP Pur
FEATURES             Location/Qualifiers
     source          1..1009
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(151..354)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(180..200)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        355..734
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tctcgccctt gctcaccatg gtggcgaccg gtagcgctag ctaggccgca gatatcgatc
       61 cgagctcggt accaagcttg ggtctcccta taggtgagtc gtattaattt cgataagcca
      121 gtaagcagtg ggttctctag ttagccagag agctctgctt atatagacct cccaccgtac
      181 acgcctaccg cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg
      241 ttgattttgg tgccaaaaca aactcccatt gacgtcaatg gggtggagac ttggaaatcc
      301 ccgtgagtca aaccgctatc cacgcccatt gatgtactgc caaaaccgca tcaccatggt
      361 aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta
      421 ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg
      481 catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg
      541 acgtcaatgg aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa
      601 tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgcg
      661 gaactccata tatgggctat gaactaatga ccccgtaatt gattactatt aataactagt
      721 caataatcaa tgtcaacgcg tatatctggc ccgtacatcg cgaagcagcg caaaacgcct
      781 aaccctaagc agattcttca tgcaattgtc ggtcaagcct tgcctttggt tgtagcttaa
      841 attttgctcg cgcactactc agcgacctcc aacacacaag ccagggagca gatactggct
      901 taactatgcg ggcatcagag cagattgtac tggagagtgc acccataggg gaatcgggga
      961 gaatctcccc gatccgtcga acgtcaggtg ggcacttttc cggggaaat
//
