LOCUS       sequence-112610-         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bicistronic expression of Centrin-mCherry fusion and Puro resistance
            gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  TITLE     Piel Lab Plasmids
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pIRES Centrin1 Cherry Puro
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     12..30
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      173..182
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(243..262)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(244..262)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(307..510)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(336..356)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        511..890
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggggccacc cttggtcacc ttcagcttgg cggtctgggt gccctcgtag gggcggccct
       61 cgccctcgcc ctcgatctcg aactcgtggc cgttcacgga gccctccatg tgcaccttga
      121 agcgcatgaa ctccttgatg atggccatgt ttatcctcct cgcccttgct caccatggtg
      181 gcgaccggta gcgctagcta ggccgcagat atcgatccga gctcggtacc aagcttgggt
      241 ctccctatag tgagtcgtat taatttcgat aagccagtaa gcagtgggtt ctctagttag
      301 ccagagagct ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa
      361 tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact
      421 cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg
      481 cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat
      541 gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca
      601 tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc
      661 caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg
      721 cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca
      781 gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac
      841 taatgacccc gtaattgatt actattaata actagtcaat aatcaatgtc aacgcgtata
      901 tctgggcccg tacatcgcga agcagcgcaa aacgcctaac cctaagccag attcttcatg
      961 caattgtcgg gtcaagcctt gcc
//
