LOCUS       sequence-112613-         660 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bicistronic expression of LifeAct-mCherry fusion and Hygro 
            resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 660)
  TITLE     Piel Lab Plasmids
REFERENCE   2  (bases 1 to 660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pIRES Hygro3 LifeAct-mChe
FEATURES             Location/Qualifiers
     source          1..660
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          342..571
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     554..573
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 ctctcgagcg gatgtacccc gaggaggcgc cctgaagggc gagatcaagc agaggctgaa
       61 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc
      121 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga
      181 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
      241 ggacgagctg tacaagtgag gatccgatat cgtacgttaa catagataac tgatccagtg
      301 tgctggaatt aattcgctgt ctgcgagggc cagctgttgg ggtgagtact ccctctcaaa
      361 agcgggcatg acttctgcgc taagattgtc agtttccaaa aacgaggagg atttgatatt
      421 cacctggccc gcggtgatgc ctttgagggt ggccgcgtcc atctggtcag aaaagacaat
      481 ctttttgttg tcaagcttga ggtgtggcag gcttgagatc tggccataca cttgagtgac
      541 aatgacatcc actttgcctt tctctccaca ggtgtccact cccaggtcca actgcaggtc
      601 gagcatgcat ctagggcggc cgcactagag gaattccgcc cctctccccc ccacccctct
//
