LOCUS       sequence-112616-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bicistronic expression of Utrophin-mRFP and Puro resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  TITLE     Piel Lab Plasmids
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pIRESpuro3+mRFP-Utr_CH
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(194..213)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(195..213)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(258..461)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(287..307)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        462..841
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ttcagcttgg cggtctgggt gccctcgtag gggcggccct cgccctcgcc ctcgatctcg
       61 aactcgtggc cgttcacgga gccctccatg cgcaccttga agcgcatgaa ctccttgatg
      121 acgtcctcgg aggaggccat ggtgctagct aggccgcaga tatcgatccg agctcggtac
      181 caagcttggg tctccctata gtgagtcgta ttaatttcga taagccagta agcagtgggt
      241 tctctagtta gccagagagc tctgcttata tagacctccc accgtacacg cctaccgccc
      301 atttgcgtca atggggcgga gttgttacga cattttggaa agtcccgttg attttggtgc
      361 caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac
      421 cgctatccac gcccattgat gtactgccaa aaccgcatca ccatggtaat agcgatgact
      481 aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc
      541 caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact
      601 tgatgtactg ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa
      661 gtccctattg gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg
      721 ttgggcggtc agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat
      781 gggctatgaa ctaatgaccc cgtaattgat tactattaat aactagtcaa taatcaatgt
      841 caacgcgtat atctggcccg tacatcgcga agcagcgcaa aacgcctaac cctaagcaga
//
