LOCUS       sequence-112623-        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty vector for lncRNA expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Alvarez-Dominguez JR, Bai Z, Xu D, Yuan B, Lo KA, Yoon MJ, Lim YC, 
            Knoll M, Slavov N, Chen S, Chen P, Lodish HF, Sun L
  TITLE     De Novo Reconstruction of Adipose Tissue Transcriptomes Reveals Long
            Non-coding RNA Regulators of Brown Adipocyte Development.
  JOURNAL   Cell Metab. 2015 May 5;21(5):764-76. doi: 
            10.1016/j.cmet.2015.04.003. Epub 2015 Apr 23.
  PUBMED    25921091
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2015.04.003"; journalName: "Cell Metab"; date: 
            "2015-05-5- 5"; volume: "21"; issue: "5"; pages: "764-76"
COMMENT     Sequence Label: pLncEXP
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(95..784)
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="QSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAINLC
                     VVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAVCI
                     CNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKGDV
                     SMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEHAI
                     ASGSALP"
     regulatory      786..795
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(834..886)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(864..884)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 aaatctttta ttttatctat ggctcgtact ctataggctt cagctggtga tattgttgag
       61 tcaaaactag agcctggacc actgatatct cgagttaggg caaggcggag ccggaggcga
      121 tggcgtgctc ggtcaggtgc cacttctggt tcttggcgtc gctgcggtcc tcgcgggtca
      181 gcttgtgctg gatgaagtgc cagtcgggca tcttgcgggg cacggacttg gccttgtaca
      241 cggtgtcgaa ctggcagcgc aagcggccac cgtccttcag cagcaggtac atgctcacgt
      301 cgcccttcaa gatgccctgc ttgggcacgg ggatgatctt ctcgcaggag ggctcccagt
      361 tgtcggtcat cttcttcatc acggggccgt cggcggggaa gttcacgccg tagaacttgg
      421 actcgtggta catgcagttc tcctccacgc tcacggtgat gtcggcgttg cagatgcaca
      481 cggcgccgtc ctcgaacagg aaggagcggt cccaggtgta gccggcgggg caggagttct
      541 tgaagtagtc gacgatgtcc tgggggtact cggtgaacac gcggttgccg tacatgaagg
      601 cggcggacaa gatgtcctcg gcgaagggca aggggccgcc ctccaccacg cacaggttga
      661 tggcctgctt gcccttgaag gggtagccga tgccctcgcc ggtgatcacg aacttgtggc
      721 cgtccacgca gccctccatg cggtacttca tggtcatctc cttggtcagg ccgtgcttgg
      781 actggccatg gtggcgaccg gtagcgctag cggatctgac ggttcactaa accagctctg
      841 cttatataga ccttcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggaagt
      901 tgttacgaca tttttggaaa agtcccgttg attttgggtg ccaaaaacaa acctcccatt
      961 gaacgtcaaa tgggggtgga agaacttgga aaatccccgt gagttcaaac cgcttatccc
//
