LOCUS       sequence-112625-         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV packaging vector for Cre-dependent, hSyn driven KOR DREADD 
            expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Vardy E, Robinson JE, Li C, Olsen RH, DiBerto JF, Giguere PM, 
            Sassano FM, Huang XP, Zhu H, Urban DJ, White KL, Rittiner JE, 
            Crowley NA, Pleil KE, Mazzone CM, Mosier PD, Song J, Kash TL, 
            Malanga CJ, Krashes MJ, Roth BL
  TITLE     A New DREADD Facilitates the Multiplexed Chemogenetic Interrogation 
            of Behavior.
  JOURNAL   Neuron. 2015 Apr 29. pii: S0896-6273(15)00289-5. doi: 
            10.1016/j.neuron.2015.03.065.
  PUBMED    25937170
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2015 Apr 29. pii: S0896-6273(15)00289-5. doi: 
            10.1016/j.neuron.2015.03.065."
COMMENT     Sequence Label: pAAV-hSyn-dF-HA-KORD-IRES-mCitrine
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(434..467)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(518..551)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(566..817)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(608..629)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     957..976
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 acgaccgacc ccgacccact ggacaagcac ccaaccccca ttccccaaat tgcgcatccc
       61 ctatcagaga gggggagggg aaacaggatg cggcgaggcg cgtgcgcact gccagcttca
      121 gcaccgcgga cagtgccttc gcccccgcct ggcggcgcgc gccaccgccg cctcagcact
      181 gaaggcgcgc tgacgtcact cgccggtccc ccgcaaactc cccttcccgg ccaccttggt
      241 cgcgtccgcg ccgccgccgg cccagccgga ccgcaccacg cgaggcgcga gatagggggg
      301 cacgggcgcg accatctgcg ctgcggcgcc ggcgactcag cgctgcctca gtctgcggtg
      361 ggcagcggag gagtcgtgtc gtgcctgaga gcgcagtcga gaaggtaccg gatcctctag
      421 agtcgactcc ggaataactt cgtataggat actttatacg aagttatgca gaatggtagc
      481 tggattgtag ctgctattag caatatgaaa cctcttaata acttcgtata gcatacatta
      541 tacgaagtta tggcgcgccc tatcacttgt acagctcgtc catgccgaga gtgatcccgg
      601 cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca
      661 gtttggactg gtagctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg
      721 gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga
      781 tcttgaagtt caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc
      841 tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc
      901 ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct
      961 tgtagttgcc gtcgtccttg a
//
