LOCUS       sequence-112627-        1072 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV packaging vector for Cre-dependent, hSyn driven KOR DREADD 
            expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Vardy E, Robinson JE, Li C, Olsen RH, DiBerto JF, Giguere PM, 
            Sassano FM, Huang XP, Zhu H, Urban DJ, White KL, Rittiner JE, 
            Crowley NA, Pleil KE, Mazzone CM, Mosier PD, Song J, Kash TL, 
            Malanga CJ, Krashes MJ, Roth BL
  TITLE     A New DREADD Facilitates the Multiplexed Chemogenetic Interrogation 
            of Behavior.
  JOURNAL   Neuron. 2015 Apr 29. pii: S0896-6273(15)00289-5. doi: 
            10.1016/j.neuron.2015.03.065.
  PUBMED    25937170
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2015 Apr 29. pii: S0896-6273(15)00289-5. doi: 
            10.1016/j.neuron.2015.03.065."
COMMENT     Sequence Label: pAAV-hSyn-dF-HA-KORD-IRES-mCitrine
FEATURES             Location/Qualifiers
     source          1..1072
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(50..83)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(134..167)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             181..207
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(841..852)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 aaattttgta atccagaggt tgattatcga taagcttgat atcgaattca taacttcgta
       61 tagcatacat tatacgaagt tattctttgc accattctaa agaataacag tgataatttc
      121 tgggttaagg caaataactt cgtataggat actttatacg aagttatgct agccaccatg
      181 tacccatacg atgttccaga ttacgctatg gaatccccga ttcagatctt ccgcggggag
      241 ccgggcccta cctgcgcccc gagcgcctgc ctgcccccca acagcagcgc ctggtttccc
      301 ggctgggccg agcccgacag caacggcagc gccggctcgg aggacgcgca gctggagccc
      361 gcgcacatct ccccggccat cccggtcatc atcacggcgg tctactccgt agtgttcgtc
      421 gtgggcttgg tgggcaactc gctggtcatg ttcgtgatca tccgatacac aaagatgaag
      481 acagcaacca acatttacat atttaacctg gctttggcag atgctttagt tactacaacc
      541 atgccctttc agagtacggt ctacttgatg aattcctggc cttttgggga tgtgctgtgc
      601 aagatagtaa tttccattaa ttactacaac atgttcacca gcatcttcac cttgaccatg
      661 atgagcgtgg accgctacat tgccgtgtgc caccccgtga aggctttgga cttccgcaca
      721 cccttgaagg caaagatcat caatatctgc atctggctgc tgtcgtcatc tgttggcatc
      781 tctgcaatag tccttggagg caccaaagtc agggaagacg tcgatgtcat tgagtgctcc
      841 ttgcagttcc cagatgatga ctactcctgg tgggacctct tcatgaagat ctgcgtcttc
      901 atctttgcct tcgtgatccc tgtcctcatc atcatcgtct gctacaccct gatgatcctg
      961 cgtctcaaga gcgtccggct cctttctggc tcccgagaga aagatcgcaa cctgcgtagg
     1021 atcaccagac tggtcctggt ggtggtggca gtcttcgtcg tctgctggac tc
//
