LOCUS       sequence-112630-        1036 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For expression and purification of a His-TAT-NLS-Cre fusion; HIV-TAT
            promotes cellular uptake of recombinant Cre into mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Peitz M, Pfannkuche K, Rajewsky K, Edenhofer F
  TITLE     Ability of the hydrophobic FGF and basic TAT peptides to promote 
            cellular uptake of recombinant Cre recombinase: a tool for efficient
            genetic engineering of mammalian genomes.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 Apr 2. 99(7):4489-94.
  PUBMED    11904364
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2002 Apr 2. 99(7):4489-94."
COMMENT     Sequence Label: pTriEx-HTNC
FEATURES             Location/Qualifiers
     source          1..1036
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     110..129
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        110..128
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    129..153
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        169..278
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             299..316
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
       61 ttattgtgct gtctcatcat tttggcaaag aattggatcg gaccgaaatt aatacgactc
      121 actatagggg aattgtgagc ggataacaat tccccggagt taatccggga cctttaattc
      181 aacccaacac aatatattat agttaaataa gaattattat caaatcattt gtatattaat
      241 taaaatacta tactgtaaat tacattttat ttacaatcaa aggagatata ccatgggcca
      301 tcaccatcac catcacggca tgggcgctgc aggtcgcaag aaacgtcgcc aacgtcgccg
      361 tccgcctgca ggcactagtg taagcttgaa gaagaagagg aaggtgtcca atttactgac
      421 cgtacaccaa aatttgcctg cattaccggt cgatgcaacg agtgatgagg ttcgcaagaa
      481 cctgatggac atgttcaggg atcgccaggc gttttctgag catacctgga aaatgcttct
      541 gtccgtttgc cggtcgtggg cggcatggtg caagttgaat aaccggaaat ggtttcccgc
      601 agaacctgaa gatgttcgcg attatcttct atatcttcag gcgcgcggtc tggcagtaaa
      661 aactatccag caacatttgg gccagctaaa catgcttcat cgtcggtccg ggctgccacg
      721 accaagtgac agcaatgctg tttcactggt tatgcggcgg atccgaaaag aaaacgttga
      781 tgccggtgaa cgtgcaaaac aggctctagc gttcgaacgc actgatttcg accaggttcg
      841 ttcactcatg gaaaatagcg atcgctgcca ggatatacgt aatctggcat ttctggggat
      901 tgcttataac accctgttac gtatagccga aattgccagg atcagggtta aagatatctc
      961 acgtactgac ggtgggagaa tgttaatcca tattggcaga acgaaaacgc tggttagcac
     1021 cgcaggtgta gagaag
//
