LOCUS       sequence-112631-         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For expression and purification of a His-TAT-NLS-Cre fusion; HIV-TAT
            promotes cellular uptake of recombinant Cre into mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Peitz M, Pfannkuche K, Rajewsky K, Edenhofer F
  TITLE     Ability of the hydrophobic FGF and basic TAT peptides to promote 
            cellular uptake of recombinant Cre recombinase: a tool for efficient
            genetic engineering of mammalian genomes.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 Apr 2. 99(7):4489-94.
  PUBMED    11904364
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2002 Apr 2. 99(7):4489-94."
COMMENT     Sequence Label: pTriEx-HTNC
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        21..130
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             151..168
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 attccccgga gttaatccgg gacctttaat tcaacccaac acaatatatt atagttaaat
       61 aagaattatt atcaaatcat ttgtatatta attaaaatac tatactgtaa attacatttt
      121 atttacaatc aaaggagata taccatgggc catcaccatc accatcacgg catgggcgct
      181 gcaggtcgca agaaacgtcg ccaacgtcgc cgtccgcctg caggcactag tgtaagcttg
      241 aagaagaaga ggaaggtgtc caatttactg accgtacacc aaaatttgcc tgcattaccg
      301 gtcgatgcaa cgagtgatga ggttcgcaag aacctgatgg acatgttcag ggatcgccag
      361 gcgttttctg agcatacctg gaaaatgctt ctgtccgttt gccggtcgtg ggcggcatgg
      421 tgcaagttga ataaccggaa atggtttccc gcagaacctg aagatgttcg cgattatctt
      481 ctatatcttc aggcgcgcgg tctggcagta aaaactatcc agcaacattt gggccagcta
      541 aacatgcttc atcgtcggtc cgggctgcca cgaccaagtg acagcaatgc tgtttcactg
      601 gttatgcggc ggatccgaaa agaaaacgtt gatgccggtg aacgtgcaaa acaggctcta
      661 gcgttcgaac gcactgattt cgaccaggtt cgttcactca tggaaaatag cgatcgctgc
      721 caggatatac gtaatctggc atttctgggg attgcttata acaccctgtt acgtatagcc
      781 gaaattgcca ggatcagggt taaagatatc tcacgtactg acggtgggag aatgttaatc
      841 catattggca gaacgaaaac gctggttagc accgcaggtg tagagaaggc acttagcctg
      901 ggg
//
